Plant tagging using radiofrequency identification (RFID) microchips is attractive for ornamental shrubs, such as rose (Rosa spp.), due to their high market value, wide distribution, health certification system, and numerous uses. Differently from other woody species for which methods of microchip implantation have been tested, rose tagging requires the possibility of insertion within canes of less than 10 mm diameter, such as those typically found in the rose nursery setting. Moreover, the possibility of tagging after grafting without changing common plant production procedures and aesthetic value are important considerations. Toward this aim, a new method of microchip insertion was developed. To test its effects on roses, two cultivars were subjected to a tagging procedure, and histological observations of tissues around the microchip and growth analysis of plant canes were performed. Microchip implantation did not cause xylem necrosis in 8- to 9-mm-diameter canes, but in lower diameter canes wilt of the lateral shoot and detriments in growth were observed compared with control plants. The tagged roses were tracked by a database developed for rose information, field log, and botanical sheet retrieval. Our findings suggest that rose plants can be safely tagged with a RFID microchip following suitable selection of cane calliper as early as the nursery phase without negative effects on plant appearance.
Andrea Luvisi, Alessandra Panattoni, Roberto Bandinelli, Enrico Rinaldelli, Mario Pagano, Barbara Gini, Giorgio Manzoni and Enrico Triolo
Manuel Di Vecchi Staraz, Roberto Bandinelli, Maurizio Boselli, Patrice This, Jean-Michel Boursiquot, Valérie Laucou, Thierry Lacombe and Didier Varès
Genetic structuring and parentage analysis were performed on a very large database comprising 2786 unique multilocus genotypes [20 nuclear simple sequence repeats (nSSRs)] of Vitis vinifera L. ssp. sativa (DC.) Hegi with a special focus on Tuscan cultivars to reveal the parentage and history of the cultivar Sangiovese, the most important cultivar of Italy. For this cultivar, the authors also analyzed clones and synonyms, investigating its genetic origin and intracultivar diversity. Known synonyms of ‘Sangiovese’ were confirmed and new ones were revealed with cultivars outside Tuscany. Some synonyms were invalidated, and unexpected homonyms were identified. The absence of true intracultivar variability leads to the rejection of a polyclonal origin for ‘Sangiovese’. The existence of an Italian genetic pool composed of ancient cultivars including Sangiovese was demonstrated by analyzing the entire set of 2786 cultivars. Ten individuals compose the kin group of ‘Sangiovese’, including two offspring: ‘Ciliegiolo’ and ‘Catarratto bianco faux’. Despite the large presence and long history of ‘Sangiovese’ in the Tyrrenian area, its kin group is unexpectedly composed of a majority of ancient cultivars that are largely diffused in far southern Italy, which leads to the hypothesis of a Sicilian origin for ‘Sangiovese’. Analysis of the Tuscan pool revealed large kin groups for cultivars Mammolo and Garganega, demonstrating their contribution to the genetic diversification in the Tyrrenian area. This work contributes to the understanding of grapevine diversification, evolution, and history in Italy and Europe.