Lobularia maritima (L.) Desv. is an important ornamental plant. We investigated an efficient method to induce tetraploid plants of L. maritima (L.) Desv. by treating germinating seeds and apical growing points of seedlings with a range of concentrations of colchicine for different periods of time. Examination of the ploidy level by counting chromosome numbers at metaphase confirmed that the chromosome number of diploid plants was 2n = 2x = 24, whereas 2n = 4x = 48 was observed in tetraploid plants. The morphological characteristics of the diploid and colchicine-induced tetraploid plants were compared. Increases in the size of leaves, flowers, and stomata were observed in the tetraploid plants compared with the diploids. However, the stomatal density and plant height of the tetraploid plants were lower than for the diploid plants. This study presents the first report of autotetraploid plants of L. maritima (L.) Desv., and of the successful generation of tetraploid plants with improved ornamental traits by colchicine treatment.
Renwei Huang, Daofeng Liu, Min Zhao, Zhineng Li, Mingyang Li and Shunzhao Sui
Daofeng Liu, Jing Ma, Jianfeng Yang, Tien V. Nguyen, Huamin Liu, Renwei Huang, Shunzhao Sui and Mingyang Li
Wintersweet is a woody ornamental plant and has a long history of human cultivation. Few molecular markers have been characterized and remain scant in wintersweet. This study aimed to mine simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs) from the transcriptomic database of wintersweet. A total of 3972 SSRs and 97,060 putative SNPs/indels (92,307 SNPs and 4753 indels) were identified in this data set. This study marks the highest number of SSR and SNP markers discovered to date from wintersweet by using transcriptome sequencing data. These identified markers will provide a useful source for molecular genetic studies such as genetic diversity and characterization, association mapping, and map-based gene cloning in wintersweet.