Search Results

You are looking at 1 - 10 of 40 items for

  • Author or Editor: Rebecca L. Darnell x
  • Refine by Access: All x
Clear All Modify Search
Free access

Rebecca L. Darnell

Containerized `Climax' and `Beckyblue' rabbiteye blueberry plants (Vaccinium ashei Reade) were exposed to 5 weeks of natural daylengths (i.e. gradually decreasing daylengths from 12 to 11 hr) or shortened daylengths (i.e. gradually decreasing daylengths from 10 to 8 hr) starting October 1. `Beckyblue' initiated twice as many flower buds under short days compared to longer days. The following spring, `Beckyblue' plants exposed to shortened photoperiods the previous fall had a greater percentage of floral budbreak (based on the number of flower buds formed within each treatment) and a shorter, more concentrated bloom period than did plants exposed to longer photoperiods the previous fall. Fresh weight per berry increased following the short fall photoperiod treatment, despite the fact that fruit number was higher. `Climax' did not respond to the photoperiod treatments in any way. Leaf carbon assimilation rates of both cultivars increased under short days, but there was no detectable effect of photoperiod on current carbon partitioning in either cultivar, suggesting that flower bud initiation is not limited by current source leaf assimilate supply under these conditions.

Free access

Rebecca L. Darnell

Containerized `Climax' and `Beckyblue' rabbiteye blueberry plants (Vaccinium ashei Reade) were exposed to 5 weeks of natural daylengths or shortened daylengths starting 30 Sept. `Beckyblue' plants exposed to short daylengths in the fall initiated more flower buds and had a shorter, more concentrated bloom period than did plants exposed to natural fall daylengths. Reproductive development of `Climax' was not influenced by photoperiod treatments. Leaf carbon assimilation of both cultivars increased under short days. Partitioning of translocated 14C-labeled assimilates to stem tissue increased under short photoperiods for `Beckyblue'; however, partitioning patterns in `Climax' were not affected. Increased carbon fixation and increased partitioning of carbon to stem tissue under short days may contribute to the observed effect of short days on enhancing reproductive development in `Beckyblue'.

Free access

Umpika Poonnachit and Rebecca L. Darnell

Vaccinium corymbosum, one of the cultivated blueberry species, is not well-adapted to mineral soils, which are generally marked by high pH, the predominance of NO3-N over NH4-N, and limited iron availability. A wild species, V. arboreum, grows naturally on mineral soils, and thus may be better adapted than V. corymbosum. This adaptation may be related to the ability of V. arboreum to assimilate NO3 and/or iron more efficiently than V. corymbosum. Both species were grown in a hydroponic solution containing 5.0 mM N as (NH4)2SO4 or NaNO3, and buffered to pH 5.5. Nitrate reductase (NR) and iron reductase (FeR) activities were measured. NR activity was higher in V. arboreum compared with V. corymbosum when grown with N03-N, while no difference between species was observed when grown under NH4-N. Activity of FeR was higher in V. arboreum compared with V. corymbosum, and higher under NO3-N compared with NH4-N. After 5 months in hydroponics, Fe was removed from one-half of the solutions. The activity of NRA in both species was higher under Fe-sufficient compared with Fe-limited conditions, but in both cases, activity was higher in V. arboreum compared with V. corymbosum. FeR activity continued to be higher in V. arboreum compared with V. corymbosum, and under NO3 compared with NH4-N. Activity decreased in both species under limited Fe conditions, and there were no interactions between species and Fe. These data indicate that V. arboreum possesses higher NR and FeR activities than V. corymbosum, under both Fe-sufficient and Fe-limited conditions. This may play a role in the better adaptability of V arboreum to mineral soil conditions.

Free access

Keith T. Birkhold and Rebecca L. Darnell

The relative contribution of storage and currently assimilated N to reproductive and vegetative growth of `Bonita' and `Climax' rabbiteye blueberry (Vaccinium ashei Reade) was estimated immediately before and during the fruit development period. Total and storage N decreased in roots and shoots of both cultivars during dormancy and early fruit development. The principle N storage form appeared to be protein, as indicated by a significant decline in total shoot and root protein during this same period. Storage N from roots and shoots in both cultivars was remobilized to flowers and/or fruit and new vegetative growth. At anthesis, 90% of the total N present in reproductive organs was estimated to come from storage N. By fruit maturity, ≈ 50% of the accumulated N was derived from storage pools. Storage N contributed 90% of the total N in developing vegetative growth of `Bonita' at leaf budbreak, which is concomitant with floral budbreak for this cultivar. Developing vegetative growth of `Climax' at leaf budbreak, which occurs ≈ 4 weeks after floral budbreak, derived ≈ 65% of its total N from storage and 35% from currently assimilated N. By fruit maturity, contribution of storage N to new vegetative growth had decreased to ≈ 20% in both cultivars, indicating that currently assimilated N became the principal N supply as vegetative growth became more established. Differences in timing of floral and vegetative budbreak between the two cultivars did not appear to affect allocation of either storage or currently assimilated N to new vegetative or reproductive growth.

Free access

Rebecca L. Darnell and Keith B. Birkhold

Rabbiteye blueberry (Vaccinium ashei Reade) cultivars differ in timing of floral and vegetative budbreak and in final fruit size. For example, `Bonita' exhibits concomitant floral and vegetative budbreak and has relatively large fruit size, while floral budbreak precedes vegetative budbreak in `Climax' and fruit size is smaller. Mobilization of carbohydrate before and during fruit development in `Bonita' and `Climax' rabbiteye blueberries was examined to determine if differences in carbohydrate availability between these two cultivars were correlated with differences in fruit size. Root dry mass (DM) of both cultivars decreased from dormancy (31 days before anthesis) through fruit development. Sugar concentrations in roots and stems of both cultivars decreased significantly between dormancy and anthesis, then remained relatively steady throughout fruit development. Starch concentrations in roots and stems of `Bonita' decreased significantly between dormancy and anthesis. The extent of total starch depletion in `Climax' was similar; however, the decrease was more gradual, extending from dormancy to 28 days after anthesis (DAA); at which time, vegetative budbreak in `Climax' occurred. Thus, although total reserve carbohydrate pool sizes were similar between the two cultivars, remobilization patterns were different, resulting in increased starch mobilization in `Bonita' compared to `Climax' in the period leading up to anthesis. Concentration of 14C from reserve carbon sources was similar in flowers of both cultivars at anthesis. These values declined throughout fruit development as a result of dilution of the labeled carbon by unlabeled carbon from current photosynthesis. There was a sharper decline in 14C concentration of `Bonita' fruit compared to `Climax' fruit between anthesis and 51 DAA. This, coupled with differences in timing of vegetative budbreak between the two cultivars, suggests that `Bonita' fruit were accessing current (unlabeled) assimilate earlier (i.e., before 51 DAA) than `Climax' fruit. Smaller fruit size in `Climax' compared to `Bonita' may be a consequence of a decrease in reserve carbohydrate mobilization to `Climax' flower buds before anthesis relative to `Bonita', as well as a delay or reduction in the availability of current carbohydrates to developing `Climax' fruit between anthesis and 51 DAA.

Free access

Rebecca L. Darnell and Steven A. Hiss

Most Vaccinium species have narrow soil adaptation and are limited to soils that have low pH, high available iron (Fe), and nitrogen (N) primarily in the ammonium (NH4+) form. Vaccinium arboreum Marsh. is a wild species that can tolerate a wider range of soil conditions, including higher pH and nitrate (NO3-) as the predominant N form. This wider soil adaptation may be related to the ability of V. arboreum to acquire Fe and NO3- more efficiently than cultivated Vaccinium species, such as V. corymbosum L. interspecific hybrid (southern highbush). Nitrate and Fe uptake, and nitrate reductase (NR) and ferric chelate reductase (FCR) activities were compared in these two species grown hydroponically in either 1.0 or 5.0 mm NO3-. Nitrate uptake rate (on a whole-plant and FW basis) and root NR activity were significantly greater in V. arboreum compared with V. corymbosum. Iron uptake on a FW basis was also greater in V. arboreum, and was correlated with higher root FCR activity than was found in V. corymbosum. Increased Fe and NO3- uptake/assimilation in V. arboreum were reflected in increased organ and whole-plant dry weights compared with V. corymbosum. Vaccinium arboreum appears to be more efficient in acquiring and assimilating NO3- and Fe than is the cultivated species, V. corymbosum. This may partially explain the wider soil adaptation of V. arboreum.

Free access

Peter A.W. Swain and Rebecca L. Darnell

Two cultivars of southern highbush blueberry (Vaccinium corymbosum interspecific hybrid) were grown in containers under the traditional deciduous production system, or the dormancy-avoiding evergreen production system. In the dormancy-avoiding system, plants are maintained evergreen and do not enter dormancy in the winter. This alleviates the chilling requirement, thus extending the potential growing area of blueberries into subtropical regions. Plants in the evergreen production system were maintained in active growth through weekly or biweekly N fertilization (≈21–23 g N/ plant per year). Keeping foliage through the year lengthens the duration of the photosynthetic season of the plant and is hypothesized to improve the carbohydrate (CHO) status of the evergreen plants. This, in turn, may decrease source limitations to reproductive development and potentially increase fruit number and/or size. In both cultivars, the evergreen production system advanced the time of anthesis by 3 to 4 weeks compared to the deciduous production system. Plants in the evergreen system initiated 10% to 25% more flower buds than plants in the deciduous system, depending on cultivar. Average leaf area, leaf fresh weight, total above-ground fresh weight, bud density, and cane length were greater in the evergreen plants than deciduous. The evergreen production system increased plant fresh weight and flower bud number compared to the deciduous system, and may ultimately increase yield.

Free access

Rebecca L. Darnell and Frederick S. Davies

Potted `Tifblue', Woodard', and `Climax' rabbiteye blueberry plants (Vaccinium ashei Reade) were exposed to artificial or natural chilling regimes (< 7C) ranging from 100 to 1000 hours during the dormant season to determine the effects on budbreak and fruit set. Insufficient chilling increased the days to 50% vegetative and floral budbreak in all three cultivars. The amount of floral budbreak increased in `Tifblue' and `Woodard', but decreased in `Climax' as chilling increased. Insufficient chilling did not decrease percent fruit set of hand-pollinated flowers in any cultivar, indicating that the fruit-setting potential of these cultivars is unrelated to chilling.

Free access

Rebecca L. Darnell and Gary W. Stutte

Strawberries (Fragaria xananassa Duch. .Osogrande.) were grown hydroponically with three NO3-N concentrations (3.75, 7.5, or 15.0 mM) to determine effects of varying concentration on NO3-N uptake and reduction rates, and to relate these processes to growth and fruit yield. Plants were grown for 32 weeks, and NO3-N uptake and nitrate reductase (NR) activities in roots and shoots were measured during vegetative and reproductive growth. In general, NO3-N uptake rates increased as NO3-N concentration in the hydroponics system increased. Tissue NO3-. concentration also increased as external NO3-N concentration increased, reflecting the differences in uptake rates. There was no effect of external NO3-N concentration on NR activities in leaves or roots during either stage of development. Leaf NR activity averaged ~360 nmol NO2 formed/g fresh weight (FW)/h over both developmental stages, while NR activity in roots was much lower, averaging ~115 nmol NO2 formed/g FW/h. Vegetative organ FW, dry weight (DW), and total fruit yield were unaffected by NO3-N concentration. These data suggest that the inability of strawberry to increase growth and fruit yield in response to increasing NO3-N concentrations is not due to limitations in NO3-N uptake rates, but rather to limitations in NO3 - reduction and/or assimilation in both roots and leaves.

Full access

Rebecca L. Darnell and Jimmy G. Cheek

Graduate student enrollment in the plant sciences has decreased over the past several years, and there is increasing interest in recruitment/retention strategies. Before successful strategies can be implemented, however, the status of current plant science graduate programs needs to be determined. Survey data on graduate student demographics, research area, support levels, current recruitment strategies, and career opportunities were collected from 23 plant science graduate programs. Overall, 55% of graduate students in plant sciences were male and 45% were female; approximately 60% were domestic and 40% were international. Cellular/molecular biology and breeding/genetics were the two disciplines that had the greatest number of graduate students and the greatest number of job opportunities. Although most programs cited financial support as the biggest obstacle to recruitment, there was not a strong correlation between graduate student number/program and stipend amount. However, other funding factors besides stipend amount; such as stipend number, the guarantee of multiple years of support, the funding of tuition waivers, and health insurance costs, likely impact student number. As more of these costs are shifted to faculty, there appears to be an increasing inability and/or reluctance to invest grant funds (which support 60% of the plant science graduate students) in graduate student education. These data suggest that the decline in plant science graduate student enrollment may not be directly due to low stipend amounts, but rather to shifting of more of the total cost of graduate training to faculty, who may be unable/unwilling to bear the cost. There is also a clear shift in the research focus of plant science graduate students, as postdoctoral and career opportunities are weighted towards molecular biology/genetics, leaving the more applied plant science areas particularly vulnerable to low graduate enrollment.