Methyl salicylate (MeSA) and Methyl jasmonate (MeJA) treatments increased chilling resistance of light red tomato (Lycopersicon esculentum cv. Beefsteak) and extended shelf life and fresh-cut quality. We previously showed induction of AOX expression by low temperature and that induction of AOX transcript by MeSA and MeJA is correlated with resistance against chilling injury in peppers. Here, we investigate tomato, which is genetically closely related to peppers and belongs to the same Solanaceae family. In particular, we used four EST tomato clones of AOX from the public database that belong to two distinctly related families, 1 and 2 defined in plants. Three clones designated as LeAOX1a, 1b and 1c and the fourth clone as LeAOX2. Probes for these four genes were designed and Southern blotting done to confirm that they do not cross-hybridize. We will present data from Southern, Northern hybridization and RT-PCR to show: (1) gene copy number of each of these AOX members in the tomato genome; (2) gene-specific expression profiles in response to MeSA and MeJA in cold stored tomato; and (3) the relative transcript abundance of these four AOX genes.
Eighteen strawberry genotypes were evaluated for their phenolic content and antioxidant capacity using several methods. High antioxidant capacity was found for `Harmonie', `Saint-Jean d'Orléans', and `Saint-Laurent d'Orléans', which were reported to have better shelf life than `Kent'. `Harmonie', `Saint-Jean d'Orléans', `Orléans', and some advanced selections had higher hydroxycinnamic acids, benzoic acids, and flavonols than `Kent'. The significant variation in antioxidant capacity and total phenolic compounds clearly shows the potential value of certain new cultivars and advanced lines as parents in a breeding program. The future plan is to examine individual antioxidant and their role in disease resistance and extension of shelf life and to use selected genotypes as parents to developed new lines.
`Clé des Champs' is a new June-bearing strawberry cultivar (Fragaria ×ananassa Duch.) bred for Eastern Central Canada and climates similar to Quebec conditions. `Clé des Champs' was released for pick-your-own and shipping because it has very attractive light red, glossy (Fig. 1), and firm fruit, which have an excellent shelf life compared with `Kent'.