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  • Author or Editor: Ralph A. Dean x
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Two 24-mer primers, MUSKFOM I and MUSKFOM II, were developed that amplify a 1.5-kb DNA fragment in race 1 Fusarium wilt resistant muskmelon (Cucumis melo L.), but not in race 1 susceptible germplasm tested. Three race 1 resistant cultivars and two race 1 resistant breeding lines as well as eight race 1 susceptible lines were analyzed using the two sequence-specific primers in the polymerase chain reaction. These primers should prove valuable for nondestructive determination of Fom 2 gene introgression in breeding programs.

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A genetic linkage [randomly amplified polymorphic DNA (RAPD)-based] map was constructed for watermelon [Citrullus lanatus (Thunb.) Matsum and Nakai] using a BC1 population [PI 296341-fusarium wilt resistant × New Hampshire Midget (fusarium susceptible)] × `New Hampshire Midget'. The map contains 155 RAPD markers, and a 700-base pair sequenced characterized amplified region (SCAR) marker that corresponds to a fragment produced by the RAPD primer GTAGCACTCC. This marker was reported previously as linked (1.6 cM) to race 1 fusarium wilt resistance in watermelon. The markers segregated to 17 linkage groups. Of these, 10 groups included nine to 19 markers, and seven groups included two to four markers. The map covers a genetic linkage distance of 1295 cM. Nine of the 10 large linkage groups contained segments with low (or no) level of recombination (0 to 2.6 cM) among markers, indicating that the watermelon genome may contain large chromosomal regions that are deficient in recombination events. The map should be useful for identification of markers linked closely to genes that control fruit quality and fusarium wilt (races 1 and 2) resistance in watermelon.

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