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A Simple and Rapid Screening Procedure for Identification of Zygotic Citrus Seedlings Among Crosses of Certain Taxa
1
Abstract
In progenies from crossing browning and nonbrowning Citrus taxa, browning was dominant to nonbrowning with certain exceptions. Examining the color of spots formed by pouring homogenates of ground young shoots on blotting paper was the simplest and most rapid procedure for scoring progenies. Presence of enzyme activity and substrate was determined by adding partially purified enzyme and substrate to the spots. Because of taxon-specificity of spot colors, it was concluded that nucellar and zygotic progenies from crosses among polyembryonic taxa could be identified at an early seedling stage, if parents with contrasting spot color were used in crosses.