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The cold hardiness of Magnolia grandiflora `Claudia Wannamaker' and `Little Gem' was determined under 8, 12 and 16 hour daylengths. Temperature was maintained at 25C day and 20C night. In addition, specific and total carbohydrates of both cultivars were analyzed. Cold hardiness and carbohydrate content were tested at the beginning (0 week), middle (5 week), and end (9 week) of the study. As expected, both southern magnolia cultivars were more cold hardy after 9 weeks at 8 hour daylengths with -9C cold hardiness estimates, as compared to 12 and 16 hour daylengths. The 12 and 16 hour daylengths resulted in similar cold hardiness estimates of -6C after 9 weeks. Additional cold hardiness and carbohydrate information will be presented.
Cold hardiness and carbohydrate content of 4 cultivars of field-grown southern magnolia (Magnolia grandiflora L.) were determined monthly during the 1992-1993 winter. Initially, `Claudia Wannamaker', `Little Gem', `Timeless Beauty', and `Victoria' had similar stem and leaf cold hardiness estimates of -6C in October. However, by February `Claudia Wannamaker' and `Victoria' stems were 6 and 3C more cold hardy than `Little Gem' and `Timeless Beauty' stems. `Claudia Wannamaker' leaves were also 6C more cold hardy than `Little Gem' and `Timeless Beauty' leaves in February. Carbohydrate analysis indicates increases in oligosaccharides during cold acclimation in fall.
Leaf water status, carbohydrate levels, net photosynthesis, stomatal conductance, ABA, dihydrozeatin riboside (DHZR), and trans-zeatin riboside (ZR) levels were determined in a greenhouse during rooting of stem cuttings of Acer rubrum L. `Red Sunset' taken on 3 Sept. 1987 and 28 May 1988. Leaf water status deteriorated before rooting and improved after root emergence. Leaf carbohydrate concentrations (glucose, sucrose, total soluble sugars, and total carbohydrates) increased until rooting and decreased after rooting, while changes in starch concentrations were trendless. ABA levels increased after insertion of cuttings into the rooting medium, but decreased before rooting. No correlation between timing of rooting and concentrations of the cytokinins ZR or DHZR was observed. Photosynthetic rates during rooting were higher for the Sept. 1987 cuttings and did not decrease to the compensation point as did those for May 1988 cuttings. Low photosynthetic rates and stomatal conductance of the cuttings during rooting were associated with water stress. The relationship between photosynthetic rates of such cuttings and cytokinin (CK) or ABA content was unclear. Chemical names used: [S-(Z,E]-5-(1-hydroxy-2,6,6-trimethyl-4-oxo-2-cyclohexen-1-yl)-3-methyl-2, 4-pentadienoic acid (abscisic acid, ABA); 2-methyl-4-(1H-purin-6-ylamino)-2-buten-1-ol (zeatin, Z).