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Abstract
Rapid in vitro propagation of Anigozanthos manglesii D. Don. was achieved through the initiation and proliferation of shoots from lateral bud explants using a modified Murashige and Skoog medium, or Gamborg, Miller and Ojima B5 medium, supplemented with 0.5 ppm benzylaminopurine (BA) and 0.5 ppm naphthaleneacetic acid (NAA). Continued proliferation was obtained by sub-culture either on the same media or media containing 0.25 ppm BA plus 0.25 ppm NAA. Rapid rooting of shoots occured following transfer to a half strength B5 medium containing 0.2 ppm NAA. Plantlets were successfully transplanted onto a University of California (UC) potting mix under mist and reduced light intensity. The method has also been successfully employed with Anigozanthos flavidus Redoute and with Macropidia fulginosa (Hook.) Druce.