Search Results
Abstract
Rapid clonal propagation of papaya (Carica papaya L.) was obtained by culturing apices of mature field-grown plants on a modified Murashige and Skoog formulation. Individual plantlets were first induced on a medium with 50 μ m kinetin and 10 μ m naphthaleneacetic acid (NAA). Plantlets were transferred after 2 months to a proliferation medium containing μ m benzyladenine (BA) and 1 μ m NAA, which caused a 7-fold increase in the total number of plants during each 3 week period. Rooting was induced by subculturing plantlets on media with 0.5 – 15 μ m indolebutyric acid (IBA) or 1–5 μ m NAA. Successful transfer of papayas to soil was accomplished.
Abstract
In vitro propagation of 2 selections of white-fleshed sweet potato (Ipomoea batatas (L.) Lam) was obtained on modified Murashige and Skoog media using lateral buds and shoot apices as primary explants. Cultivars differed in response to exogenous levels of growth substances and in rate of proliferation. Optimum shoot regeneration from ‘White Star’ explants was induced by 1 mg/liter benzyladenine (BA) and from Plant Introduction (PI) 315343 by 1 mg/liter kinetin with 1 mg/liter indoleacetic acid (IAA).
Abstract
Leaf disks of Hausa potato (Coleus parviflorus Benth.) were cultured on Murashige and Skoog (MS) medium. Optimum adventitious shoot development on the explant was obtained using 6.0 to 8.0 mg/liter 6-benzylamino purine (BA) in combination with 2.5 to 4.0 mg/liter naphthaleneacetic acid (NAA). Shoot regeneration was obtained from callus following callus subculture on media with 4.0 to 8.0 mg/liter BA in combinations with 1.5 to 4.0 mg/liter NAA. Rooting was induced on shoots by transfer to a growth regulator-free medium.
Abstract
Shoot tips of Xanthosoma caracu were cultured and rooted successfully in 5 to 6 weeks in an agitated liquid Murashige and Skoog (MS) basal medium with 100 mg/liter ascorbic acid and multiplied in MS basal medium with (per liter) 250 mg NaH2PO4 · H2O, 0.4 mg thiamine-HCl, 30 mg adenine sulphate, 8 g Difco Bacto agar and supplemented with 2 mg benzylaminopurine (BA), and 0.5 mg indoleacetic acid (IAA). Multiplication rate was 45 to 70 plants per culture flask within 9 to 10 weeks after subculture.
Abstract
Bulbil explants of one Dioscorea bulbifera L. and two D. alata L. genotypes were cultured on Murashige and Skoog (MS) medium which was modified to contain 500 mg/liter NH4NO3 as the only N source. Factorials involving kinetin, 2,4-dichloro-phenoxyacetic acid (2,4-D), benzylamino purine (BA), and naphthaleneacetic acid (NAA) were tested. Plant development was obtained from one D. alata genotype using 10.0 to 14.0 mg/liter kinetin in combination with 0.01 to 0.02 mg/liter 2,4-D.
Abstract
Isozyme markers for glutamate oxaloacetate transaminase (GOT), superoxide dismutase (SOD), peroxidase (PER), and malate dehydrogenase (MDH) were identified for Carica papaya L. and the related but sexually incompatible C. cauliflora Jacq. These markers were used to determine the nature of somatic embryos derived from papaya ovules cultured on modified Murashige and Skoog (MS) medium 65 days after controlled pollination with C. cauliflora. Zymograms of plantlets from somatic embryos contained bands specific to either C. papaya or C. cauliflora (PER, GOT) and a unique band not present in the zymogram of either species (PER). Zymograms of somatic embryo-derived plantlets were distinctively different from those of either of the Carica species for all the enzyme systems examined. Evidence from isozyme markers indicates that somatic embryos produced from cultured papaya ovules following pollination with C. cauliflora may be hybrids. The isozyme banding patterns of 60 plantlets derived from somatic embryos from the same ovule were very uniform and suggest genetic uniformity among the regenerated plantlets.
Abstract
‘Cariflora’ is a dioecious papaya (Carica papaya L.) cultivar, tolerant of infection by papaya ringspot virus (PRV), that has been developed for south Florida and the lowland Caribbean region. ‘Cariflora’ produces good quality, round fruit with sweet yellow flesh and an agreeable aroma. This papaya is particularly suitable for commercial plantings because of the small size of its fruit, but it also could be useful to home growers. ‘Cariflora’ is being released for grower trial by the Agricultural Experiment Stations of the Univ. of Florida.
Abstract
Excised zygotic embryos of Christmas palm [Veitchia merrilli (Bacc.) H.E. Moore] developed large haustoria and germinated normally in vitro on Murashige and Skoog (MS) medium plus 0.25% activated charcoal, 5-50 µm 2,4-D and 5 µm BA. Embryogenic callus was induced from mature zygotic embryos when they were cultured on charcoal-free MS medium supplemented with 170 mg/liter KH2PO4, 200-400 mg/liter glutamine, and 5-25 µm 2,4-D. Somatic embryos developed and matured in a hormone-free, glutamine-containing medium. Plantlets developed from somatic embryos on MS basal medium or MS plus 5 µm NAA, 5 µm 2iP, and 2.5 µm GA3. Embryogenic calli have been maintained on MS medium for more than 6 months. Chemical names used: (2,4-dichlorophenoxy)acetic acid (2,4-D); N-(phenylmethyl)-1Hpurin-6-amine (BA); 1-naphthaleneacetic acid (NAA); N-(3-methyl-2-butenyl)-1H-purin-6-amine (2iP).