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  • Author or Editor: R. Cowen x
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The only method for large scale production of pure hybrid seed in Zinnia elegans involves the use of male sterile individuals. The male sterile trait, however, is a three gene recessive which at best produces only 50% male sterile progeny from seed. Since no method of clonal propagation is available, seed-produced female lines require labor intensive field roguing to insure removal of all normal flowered individuals. Clonal micropropagation was investigated as a means of mass producing male steriles for use as female lines. Sterilization procedures were developed for seed and axillary bud explants. Shoot proliferation media containing various levels of BAP, 2ip, and kinetin were screened using in vitro germinated seedling explants of the inbred line `Orange Starlight'. Microshoots demonstrated a high rooting percentage after 2 weeks on basal medium without growth regulators. Plantlets were easily acclimated in 1 to 2 weeks in a high humidity environment. In vitro derived plants of identified male sterile plants were phenotypically evaluated as to their suitability for use in field production.

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The only method for large scale production of pure hybrid seed in Zinnia elegans involves the use of male sterile individuals. The male sterile trait, however, is a three gene recessive which at best produces only 50% male sterile progeny from seed. Since no method of clonal propagation is available, seed-produced female lines require labor intensive field roguing to insure removal of all normal flowered individuals. Clonal micropropagation was investigated as a means of mass producing male steriles for use as female lines. Sterilization procedures were developed for seed and axillary bud explants. Shoot proliferation media containing various levels of BAP, 2ip, and kinetin were screened using in vitro germinated seedling explants of the inbred line `Orange Starlight'. Microshoots demonstrated a high rooting percentage after 2 weeks on basal medium without growth regulators. Plantlets were easily acclimated in 1 to 2 weeks in a high humidity environment. In vitro derived plants of identified male sterile plants were phenotypically evaluated as to their suitability for use in field production.

Free access

Abstract

Two narrow-leaf (N1 and N2) and two wide-leaf (W1 and W2) Zinnia elegans Jacq. lines were developed. Three crosses were made: N2 × W2, W1 × N2, and W2 × N1. Six generations (P1, P2, F1, F2, BC-P1, and BC-P2) for each of the three crosses were evaluated on the basis of leaf width. A generation means analysis indicated that additive gene effects were of significant importance in the control of leaf width, while both dominance and epistatic effects were nonsignificant. High gains from selection on the basis of leaf shape are, therefore, indicated.

Open Access