Search Results

You are looking at 1 - 3 of 3 items for

  • Author or Editor: Phillip T. Evans x
Clear All Modify Search

Abstract

A single rhizome explant of the Venus fly-trap has the potential to produce 14 or more rooted plantlets in 40 to 60 days when cultured on a medium containing half strength Murashige and Skoog salts, organic components, naphthaleneacetic acid (NAA) at 1.9 mg/liter and 6-benzylamino purine (BA) at 0.2 mg/liter. Cultures were grown in 16 hour cycles of Cool White fluorescent light at 23° to 26°C. Explants derived from either lateral buds or adventitious buds from leaf cuttings have equal potential for rapid multiplication. This same medium produced optimum plantlet size and quality. Supplementing the basal medium with 0.3 or 1.0 mg/liter of GA3 decreased the number of explants and increased the size of plantlets prior to acclimatization. Media containing higher and lower salt concentrations and higher and lower IAA, NAA, 2,4-dichlorophenoxyacetic acid (2,4-D), BA, or 6(γ,γ, dimethylallylamino)-purine (2ip), produced fewer plantlets while increasing deleterious effects. The rapid plantlet multiplication procedure described will increase commercial availability of the plants while decreasing collection pressures on wild germplasm pools.

Open Access

Abstract

Excised whole leaves of in vitro cultured Venus fly-trap plants produced up to 15 adventitious bud- (AB) and lateral bud-derived (LB) plantlets in 60 to 90 days, when cultured on a modified Murashige and Skoog salt medium supplemented with naphthaleneacetic acid (NAA) at 1.9 mg/liter and 6(-γ,γ-dimethylallylamino)-purine (2iP) at 0.2 mg/liter and grown under a 16 hr photoperiod at 22°C. Leaves dipped for 10 seconds to 24 hr in various concentrations of 6-benzylamino purine (BA), 2iP, NAA and indolibutyric acid (IBA) improved the production of plantlets over undipped leaves. A 24 hr 2iP dip at 2.1 mg/liter produced the largest average number of adventitious and lateral bud-dervied plantlets. Lateral bud-derived plantlets are proposed to have arisen from nodes within the rhizomes of previously derived plantlets. Morphologically, the resulting chain-like growth habit of lateral bud-derived plantlets is very unusual and provides an increased number of plantlets for subcultures.

Open Access