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  • Author or Editor: Patricia M. Buckley x
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Most bacteria isolated from persistently contaminated micropropagated mint plants were Gram-negative rods identified as xanthomonads, pseudomonads, and agrobacteria based on their cultural characteristics. A few Gram-positive, non-sporeforming bacteria were also found. Inhibition of bacterial growth by gentamicin and streptomycin was greater at pH 6.5 and pH 7.5 than at pH 5.5. Inhibition by rifampicin and Timentin was less affected by pH change. Pseudomonads were uniformly resistant to Timentin at all pH's and at levels up to 1000 μg/ml. Streptomycin at 500 μg/ml was bactericidal for the pseudomonads and Gram-positive bacteria while 1000 μg/ml was required to kill xanthomonads and agrobacteria. Minimal bactericidal concentrations for gentamicin varied widely, even within groups, and ranged from 10 μg/ml to >80 μg/ml for agrobacteria. These results emphasize a need to acquire basic information about the identities and antibiotic susceptibilities of microbial contaminants before attempting treatment of infected plant cultures.

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