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- Author or Editor: P. C. Bilkey x
Differential regeneration capacities of epidermal and sub-epidermal tissues of Saintpaulia ionantha Wendl. ‘Georgia’ were dependent on the basal salts used in the tissue culture medium. Murashige and Skoog salts medium containing 0.1 mg/liter a-naphthaleneacetic acid (NAA) and 0.5 mg/liter benzyamino purine (BA) induced callus from petiole cross-sections and similar cross-sections with their epidermis removed. Shoot regeneration occurred only on the explants containing an epidermis. B5 salts medium, with the same growth hormone concentrations, induced shoot regeneration from both explant types. When grown to flowering, plants derived from sub-epidermal tissues were more vigorous, became 25% greater in fresh weight, 11% larger in diameter, and produced 28% more leaves, which were 14% wider with petioles 18% longer, than their epidermal derived counterparts.
When optimal levels of growth substances, particularly cytokinins, are utilized, about 5,000 commercially useable plantlets of African violet (Saintpaulia ionautha Wendl.) can be generated from a single petiole in 3-4 months. Murashige and Skoog (MS) high salt medium containing 0.1 mg/liter α-naphthalene-acetic acid (NAA) and 0.01 mg/liter benzyladenine (BA) proved adequate for 2 mm petiole cross-sections.