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Abstract
Callus cultures from 2-week, 2-month, and 2-year-old Pelargonium × hortorum Bailey cv. Sprinter on modified Linsmaier and Skoog medium supplemented with various concentrations of α-naphthaleneacetic acid (NAA) and 6-furfurylaminopurine (kinetin), while anthers from 2-month and 2-year old plants were cultured on a separate medium. Fresh weight increases of callus which were measured 1, 2, and 3 weeks after plating stem explants were ×13.4, ×22.9, and ×39.0 for 2-week-old seedlings; ×4.8, ×7.1, and ×l9.0 for 2-month old plants; and ×3.5, 5.9, and ×19.5 for 2-year-old plants. Only callus from explants of 2-week-old seedlings regenerated shoots and roots after 6-8 weeks on medium containing 0.2 mg/liter NAA and 2.0 mg/liter kinetin. An average of 11.2% of ‘Sprinter’ anthers from flowers on 2-month-old plants yielded callus, whereas only 2% of anthers from 2-year-old plants yielded callus.