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- Author or Editor: Olivia C. Broome x
Abstract
Rapid propagation of the thornless blackberries (Rubus sp.) ‘Smoothstem’ and US 64-39-2 was achieved by culturing shoot tips in a modified Murashige and Skoog medium containing benzyladenine (BA), gibberellic acid (GA3) and indolebutyric acid (IBA). Shoots were explanted into liquid culture and then transferred to an agar medium for rapid shoot proliferation. “Minicuttings” from shoot-masses were rooted in vitro or directly in peat pellets under mist after auxin treatment.
Abstract
Cuttings of apple (Malus domestica Borkh.) taken from in vitro proliferated shoots growing on a modified Murashige and Skoog medium were evaluated for ability to initiate adventitious roots in the presence or absence of phloroglucinol at several concentrations of indolebutyric acid (IBA). Phloroglucinol did not promote rooting of ‘Northern Spy’, ‘Summer Rambo’ or ‘Delicious’, had a minor or inconsistent effect upon ‘Nugget’, ‘Ozark Gold’, ‘Spuree Rome’, and ‘Stayman’ and stimulated rooting of ‘Spartan’ cuttings. However, phloroglucinol did not increase rooting of ‘Spartan’ cuttings above that obtained in preliminary experiments without phloroglucinol. Autoclaved phloroglucinol produced the same responses as filter sterilized when tested on ‘Spartan’, ‘Stayman’, and ‘Summer Rambo’. Including IBA in the medium improved rooting of all cultivars and this effect was greater than the effect of phloroglucinol except for ‘Spartan’. Phloroglucinol reduced formation of callus and misshapen roots on cuttings receiving IBA treatment. There was no evidence that phloroglucinol is essential to in vitro rooting of these apple cultivar cuttings.
Abstract
A technique is described for forcing the buds of tea crabapple using cytokinins. Of the cytokinins tested, 6-benzylamino purine (BA) produced the best results at 2,000 ppm, 6-(benzylamino)-9-(2-tetrahydropyranyl)-9H-purine (PBA) was nearly as effective, and N6-[Δ2-isopentenyl]-adenosine (IPA) and N6-[Δ2 isopentenyl]-adenine (2ip) were ineffective. Depending on the cytokinin used, inclusion of 0.5 or 1.0% surfactant was required to insure consistent results. No differences were found among 8 surfactants tested. Dimethylsulfoxide (DMSO) was an excellent solvent for the cytokinins and slightly enhanced their effect. The treatment effect was not translocated from the site of application. This method was consistent, faster than 10 weeks of cold treatment, less phytotoxic than cytokinins applied in a lanolin fraction, and permitted rapid spray application of small quantities of solution.