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FaRCa1 is a major locus conferring resistance to anthracnose fruit rot (AFR) caused by Colletotrichum acutatum, an important pathogen of strawberry (Fragaria ×ananassa). The objective of this study was to characterize the effects of FaRCa1 on anthracnose root necrosis (ARN) via root inoculations and DNA marker characterization of the locus. A subgenome-specific high-resolution melting (HRM) marker for an insertion/deletion (InDel) near FaRCa1 was designed using the ‘Camarosa’ octoploid reference genome. The marker was used to genotype cultivars and advanced selections studied in two seasons. A root disease screening method was developed in which roots were cut and dipped in a spore suspension before planting, using a mixture of three local isolates of the C. acutatum species complex. ARN was indirectly scored by calculating image-based leaf area differences among inoculated and noninoculated plants. The allele of FaRCa1 conferring resistance to AFR also conferred a significant reduction in ARN. Thus, a robust and easily scored DNA test is now available to breeders for selecting for resistance to both the fruit and root forms of strawberry anthracnose.
Environmental conditions in Florida are favorable for the development and persistence of insects and diseases that affect rose (Rosa sp.) plants, necessitating periodic applications of pesticides to maintain plant appearance. In addition, nutrient-deficient and well-drained soils in Florida force gardeners to provide supplemental fertilizer and water. Landscape performance is rarely considered for the development of new rose cultivars; consequently, careful selection of cultivars adapted to local conditions is necessary to reduce maintenance. The objective of this study was to develop recommendations of own-root, low-maintenance roses among 11 old garden and modern cultivars for central Florida. Plants were provided with minimal amounts of water and fertilizer, no control for diseases and insects, and no grooming or deadheading. Weekly evaluations were performed on all plants for plant quality, flower coverage; and incidence of black spot (caused by Diplocarpon rosae), cercospora leaf spot (caused by Cercospora rosicola), and foliar damage [caused by chilli thrips (Scirtothrips dorsalis)]. Damage caused by the foliar diseases and chilli thrips were the major factors that affected plant quality, vigor, and subsequently, flower production. Differences in susceptibility to these three factors were found among cultivars, enabling the classification of the 11 cultivars as recommended, cautiously recommended, and not recommended for central Florida. After two years, ‘Mrs. B.R. Cant’ appeared to be the most suited for central Florida as plant quality and flower production were fairly constant. ‘Duchesse de Brabant’, ‘RADrazz’ (Knock Out®), and ‘Spice’ were the next best performers and are cautiously recommended for central Florida. These cultivars were minimally affected by both diseases, showing low severity of yellowing and defoliation nor a decline in flower production. “Bailey Red”, ‘Old Blush’, ‘Belinda’s Dream’, ‘Perle d’Or’, ‘BUCbi’ (Carefree Beauty™), ‘Mutabilis’, and ‘WEKcisbako’ (Home Run®) had severe defoliation, poor growth, and low vigor in this study and do not appear to be low-maintenance landscape roses for central Florida.
Bacterial blight, caused by Xanthomonas axonopodis pv. dieffenbachiae (Xad), is the most common foliar disease of caladium, an ornamental aroid grown for its colorful foliage. The disease can reduce the marketability of caladiums produced as potted plants and lower the yield of caladiums grown for tuber production. Three bacterial strains were isolated from symptomatic caladiums and identified as Xad using fatty acid analysis, carbon source use, and the sequence of the 16S-23S spacer, and tested for virulence against three susceptible cultivars. Two strains were virulent to all of the cultivars; however, one strain was differentially pathogenic, virulent against two cultivars, but not to the usually highly susceptible ‘Candidum’. In greenhouse inoculation tests of 17 cultivars and one breeding line, four cultivars were ranked as highly susceptible, nine as moderately susceptible, and five as resistant. Ten of these cultivars were also evaluated with natural infection in the field with good agreement between the results of the greenhouse and field evaluations. Cultivars White Queen, Florida Red Ruffles, Florida Sweetheart, Candidum Jr., and Mrs. Arno Nehrling have been identified as resistant to bacterial blight in greenhouse or field evaluations and can potentially be used in future breeding efforts to produce improved cultivars.