Compatible solute accumulation regulating osmotic adjustment (OA) is associated with drought tolerance. The objectives of this study were to examine genetic variations in OA among a diverse group of bentgrass (Agrostis sp.) genotypes or lines with differential drought tolerance, and determine major types of organic osmoregulants contributing to OA and accounting for the genetic variations in drought tolerance. A wild type cultivar of creeping bentgrass [Agrostis stolonifera (Penncross)], a transgenic line of creeping bentgrass (SAGIPT41), and four hybrid bentgrass lines [Agrostis capillaris × Agrostis stolonifera (ColxCr14, ColxCr190, ColxCr481, and ColxCr679)] were exposed to drought stress by withholding irrigation for 17 days in growth chambers. Among genotypes, ColxCr14, ColxCr190, and SAGIPT41 showed superior drought tolerance, as manifested by higher turf quality (TQ) and leaf relative water content (RWC), as well as OA than ‘Penncross’, ColxCr679, and ColxCr481 under drought stress. SAGIPT41 leaves accumulated greater content of soluble sugars (glucose, sucrose, and fructose), proline, glycine betaine (GB), and spermine; ColxCr190 had higher content of soluble sugars and spermidine; and ColxCr14 accumulated more soluble sugars and GB, compared with the three drought-sensitive genotypes. Soluble sugars were predominant contributors to OA, followed by GB and proline, with all three forms of polyamine (PA) as minor contributors in bentgrass genotypes. The osmolytes highly correlated to OA and superior drought tolerance could be used as biomarkers to select for drought-tolerant germplasm of bentgrass and other cool-season turfgrass species.
Nanqing Liu, Yixin Shen, and Bingru Huang
Nanqing Liu, Shaoyan Lin, and Bingru Huang
Glycine betaine (GB) and spermidine (Spd) are known to play roles in plant adaptation to stresses that induce dehydration, including drought stress. The objectives of this study were to examine whether improved drought tolerance by exogenous application of GB or Spd is associated with the increases in the endogenous accumulation of those solutes under drought stress in cool-season turfgrass species, and to determine the relative effects of those solutes on osmotic adjustment (OA) and antioxidant metabolism for improving drought tolerance. Creeping bentgrass (Agrostis stolonifera cv. Penncross) plants were treated with 200 mm GB or 0.1 mm Spd for 3 weeks by weekly foliar application before the exposure to drought stress; those plants were subsequently subjected to drought stress by withholding irrigation. The endogenous content of GB and Spd increased significantly through the exogenous application of either compound, to a greater magnitude for GB. The comparison of GB- or Spd-treated plants to untreated plants exposed to drought stress for growth [turf quality (TQ)] and physiological responses (water relations, membrane stability, and antioxidant metabolism) demonstrated that both compounds led to significant improvement in drought tolerance in creeping bentgrass. The improved drought tolerance by exogenous GB could be due to its contribution to OA by significant accumulation of endogenous GB, and activation of antioxidant enzymes with the greatest effects on ascorbate peroxidase (APX). Exogenous Spd did not cause increases in leaf OA despite of the increased endogenous accumulation, but significantly enhanced antioxidant enzyme activities, with the most pronounced effects on catalase (CAT). This study demonstrated that GB and Spd had different effects on OA and activated different antioxidant defense pathways, protecting plants from drought damages in creeping bentgrass.