Seed and seedling cotyledon explants from 14 cultivars were placed on 2 media types to induce organogenesis and somatic embryogenesis. Shoots or somatic embryos were counted to determine effects of cultivar, explant and regeneration type on overall regeneration success. Opposite explant preferences were observed for each regeneration type. In somatic embryogenesis, greater numbers of seedling cotyledons were able to respond, while in organogenesis, seed cotyledons responded in greatest numbers. However, within each cultivar, no explant preferences were observed (except in `Picklebush'). Four cultivars displayed a preference for the somatic embryogenesis regeneration protocol over organogenesis: `Burpee Hybrid II' and `Burpless F1 Hybrid' (fresh market types), and `Cross Country' and `Picklebush' (pickling types). The best individual regeneration rates were obtained with `Cross Country' and `Picklebush' - both for somatic embryogenesis.
Nancy J. Zimmerman and Nancy A. Reichert
Nancy A. Reichert and Nancy J. Zimmerman
Fourteen commercial cucumber (Cucumis sativus L.) cultivars were screened on media designed to induce somatic embryogenesis and shoot organogenesis. Cotyledon explants from mature dry seeds and 7-day-old seedlings also were compared for responses. Seven cultivars (Burpless F1Hybrid, Burpee Hybrid II, Cross Country, Picklebush. Sweet Delight, Tasty Green, and Yard Long) responded best for individual regeneration ability. Within the somatic embryogeneais regeneration protocol, `Picklebush' responded better than most other cultivars, while `Burpless F1 Hybrid' responded best in shoot organogenesis. Also, within each type of regeneration, the developmental stage of the cotyledon explants appeared to influence regeneration ability.