Callus from leaf explants of two pomegranate (Punica granatum L.) clones produced adventitious shoots after 2 months of in vitro culture. Leaf segment explants initially were cultured on a modified Murashige and Skoog (MS) basal medium supplemented with a range of 0.5-10 μm BA and 0.05-5 (μm NAA. Shoot elongation was stimulated most efficiently when the initial calli were transferred from the shoot induction medium to half-strength MS supplemented with 2 μm BA. Elongated shoots rooted easily on a medium of one-half MS and 0.1 μm NAA. Whole plants were recovered after transfer to vermiculite in a greenhouse. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA), 1-naphthaleneacetic acid (NAA).
Anthers of pomegranate (Punica granatum L.) were cultured in vitro on nutrient media containing BA and NAA. Callus from anther walls were produced after 30 days of culture. Subsequently, adventitious shoots were formed by transferring callus to a medium containing 2.0 μm of BA and 0.5 μm of NAA. Regenerated shoots had a diploid chromosome number of 18. Chemical names used: N-(phenylmethyl)-1H/purin-6-amine (BA); 1-naphthaleneacetic acid (NAA).