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Abstract
Postharvest life of cut Ranunculus asiaticus flowers was substantially extended with low temperature storage; 0° and 5°C were superior to higher temperatures. Extended storage duration, however, increased the rate at which floral senescence occurred upon removal from cold storage.
Abstract
In vitro propagated shoots of the apple rootstock, Antonovka 313 (Malus pumila Mill.)9 were rooted successfully in vitro. Roots became visible in 6-8 days, and 100% rooting after 2 weeks was achieved consistently in shoots cultured on modified Murashige and Skoog (MS) salt medium supplemented with 0.25 μm indole-3-butyric acid (IBA). Sucrose was the most influential medium component for rhizogenesis. Inorganic nutrients, IBA and vitamins did not influence rooting. Omitting activated charcoal caused only a slight decrease. A 1.5% sucrose solution added to a peat-vermiculite growing medium in vitro resulted in higher rooting than in treatments without sucrose.
Abstract
Multiple shoot formation was obtained from terminal and lateral buds excised from stems of Exacum affine on a medium containing Murashige and Skoog salts, vitamins, sucrose, agar plus 0.01 mg/liter naphthaleneacetic acid (NAA) and 2.0 mg/liter kinetin. Root formation occurred at the expense of shoot production at 1.0 mg/liter NAA plus 0.1 mg/liter kinetin. Individual shoots could be rooted directly under normal greenhouse conditions.
Abstract
An investigation of free amino acids, phenols, and indoles from various plant parts of double (DD and Dd) and single (dd) flowered petunia plants was made to determine if a relationship exists between these substances and flower genotype. There was no difference between genotypes in total free amino acid concentration from shoot tips or anthers. Flower buds with the DD genotype and leaves with the Dd genotype were significantly different in total amino acid concentration from similar tissue of the other genotypes. TLC analysis of amino acid extracts from shoot tips, buds, or leaves failed to resolve any qualitative differences between the 3 genotypes. Anthers directly squashed on TLC plates and developed in several solvent systems did not resolve any differences between genotypes. TLC analyses of phenols and indoles indicated they were very similar for the 3 genotypes.
Abstract
Water absorption curves were developed for air dry peat-vermiculite and pine bark media. Data indicated 70-78% of moisture saturation was attained within 5 days with a peatvermiculite medium while 48 days were required to achieve 58-70% saturation with a milled pine bark medium. Increased water absorption of pine bark after 10 days of wetting suggested that a threshold moisture level within the bark particles is necessary if water uptake is to be enhanced. The threshold moisture content was established at 35% (wet wt basis).
Abstract
The external surfaces and internal structures of particles of milled pine bark (Pinus taeda L. and P. elliottii Engelm.) were examined with scanning electron microscopy. Numerous external openings, cracked cell walls and internal cellular connections, that might allow water penetration were observed. Periderm surfaces were without pores, and contained rough surfaces and apparently waxy substances that might resist water penetration or absorption.