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- Author or Editor: Murray E. Hopping x
Abstract
Acidic ethyl ether-soluble growth substances extracted from sour cherry (Prunus cerasus L. cv. Montmorency) seeds were subjected to repeated paper chromatography. A growth promoting substance was isolated, active in both the Avena first internode and Avena curvature bioassays, with chromatographic and chromogenic properties similar to those of indole-3-acetic acid (IAA). This tentative identification was strengthened by gas-liquid co-chromatography of the trifluoroacetyl derivatives of authentic IAA and the endogenous growth promoter. Conclusive confirmation that the growth promoting substance is IAA was provided by combined gas chromatography-mass spectrometry.
Abstract
Endogenous acidic and neutral growth-promoting substances were extracted from sour cherry (Prunus cerasus L. cv. Montmorency) seed and pericarp tissues, separated by ascending paper chromatography and detected by the Avena first internode bioassay. Although correlations were established between the levels of seed auxin and concomitant development of the nucellus and integuments and the development of the endosperm and embryo, no correlation was found between the levels of seed and pericarp auxin or between the level of pericarp auxin and concomitant fruit development.
Abstract
Limitations in interpretation of data from 1 dimensional chromatograms is illustrated using growth substances extracted from developing seeds of sour cherry (Prunus cerasus L. cv. Montmorency).
Abstract
Ethanolic extracts of sour cherry (Prunus cerasus L. cv. Montmorency) fruit were partitioned into butanol and water-soluble fractions. Following paper chromatography, each fraction was tested for cytokinin activity by the radish cotyledon and soybean callus bioassays. Activity in both fractions increased between 14 and 21 days after anthesis, and remained high at 28 days, although cell division in the pericarp had ceased. One growth promotor in the butanol fraction resembled zeatin in its chromatographic mobility on both paper and Sephadex LH-20, and was identified as zeatin by gas chromatography-mass spectrometry of its trimethylated derivative. A water-soluble growth promoter, which exhibited activity in only the radish cotyledon bioassay, was unaffected either by acid or by alkaline phosphatase hydrolysis.