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- Author or Editor: Muhammad A. Lodhi x
A project to develop a linkage map of the Vitis genome is underway using an interspecific hybrid grape population from `Cayuga White' × `Aurore'. The linkage map is based on 15 isozyme, 13 RFLP and more than 350 RAPD markers. This information is being combined with segregation data for viticulturally important traits including disease resistance to identify QTLs and mark simply inherited traits. Molecular markers, when linked with morphological traits, will find great application in map-based cloning and marker-assisted selection in grape breeding. We have also analyzed genome size of three genera of Vitacae (Ampelopsis, Parthenocissus and Vitis) and 15 Vitis species as well as 15 diploid cultivars using flow cytometry. DNA content varied from 0.84 to 1.40 pg/2C. The relative small genome size indicates that Vitis is a good candidate for molecular genome analysis. All this information will help us understand Vitis genome organization and reliability of identifying a gene linked to a marker in different populations.