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- Author or Editor: Mu Peng x
To date, a narrow genetic base is a serious obstacle in peach (Prunus persica L.) production. Wild peach resources are useful germplasms for breeding new cultivars. In this study, amplified fragment length polymorphisms (AFLPs) were used to analyze the genetic diversity and relationships of wild and cultivated peach germplasms. These results showed that AFLP is an efficient technique for identifying the genetic relationships of wild and cultivated peach. Thirteen AFLP primer combinations generated a total of 377 scorable and clear fragments, all of which (100%) were polymorphic. Moreover, the polymorphism information content (PIC) values ranged from 0.91 to 0.96 with a mean of 0.95. The results of the principal component analysis (PCoA) largely corresponded to those obtained using cluster analysis. The three principal axes accounted for 2.6%, 5.79%, and 25.26% of the total variation, respectively. In conclusion, wild peach germplasms should receive special attention to ensure their conservation.
To analyze the evolutionary level of Prunus mira Koehne (Prunus mira Koehne Kov et. Kpst), 15 kinds of pollen grains from five altitudes were observed using a scanning electron microscope (SEM). This study demonstrates that pollen morphous P. mira has high variation; specifically, individuals from higher altitudes are much more evolved than those from lower altitudes. This is the first time the pollen morphology of P. mira has been systematically illustrated. Furthermore, 12 random amplified polymorphic DNA (RAPD) primers generated clear and repeatable bands among all individuals based on RAPD; 107 bands ranging from 200 bp to 2000 bp were generated with an average of 8.92 bands per primer. Thus, the RAPD technique proved to be a powerful tool to reveal variation on P. mira. This study provides comprehensive information for genetic diversity of P. mira from different altitudes.
Inter simple sequence repeat (ISSR) were used to evaluate the genetic diversity of Kongpo Monkshood (Aconitum kongboense L.) in Motuo, Tibet Plateau. From 70 accessions of three populations, 10 out of 100 informative ISSR primers were chosen for polymorphism analysis. Percentage of polymorphic bands was 50% to 66.67% with a mean of 58.42%. The effective number of alleles (Ne) was between 1.545 (population 3) and 1.586 (population 2), and the mean value was 1.564; the Nei’s gene diversity (h) ranged from 0.315 to 0.327 with the average value of 0.320; the value of Shannon’s information index (I) ranged from 0.459 to 0.478, with the mean of 0.469. Based on molecular data, cluster analysis classified the 70 cultivars into three groups. Most accessions were related to the geographical origin and their genetic backgrounds. Bayesian structure and PCoA analysis were consistent with the dendrogram result. Based on the analysis, it will provide a reference for Kongpo Monkshood breeding purposes and contribute to identification, rational exploitation, and conservation of germplasms.