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  • Author or Editor: Mohammed A.M. Aly x
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Squash is grown in the field and in tunnel type greenhouses in Saudi Arabia. To satisfy the demand for squash, additional production in controlled greenhouses would be desirable. The Jedida cultivar was treated with growth regulators. Seedlings were sprayed with Ethrel at 400 ppm. At flowering, six treatments were made: Agriton (60g/100L), sprayed at 10 day intervals; IAA (1%), IBA (1%), and Rootone, dusted on stigmas; hand pollination; and control. Fruits were harvested when they were 12 cm long. Ethrel increased the number of female flowers by 96% and changed the female: male ratio from 1:2 to 8:1. Highest marketable yield was obtained with the hand pollination, IBA, and IAA treatments. Yield in the Control treatment was low due to low insect activity. The results suggest that treatment with IAA or IBA will permit production of squash on a commercial scale in controlled greenhouses.

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Many members of the Plumbaginaceae are important flower crops wherein propagation is hindered by poor seed germination. Micropropagation via organogenesis is commercially practiced for certain Limonium species. However, somatic embryogenesis was not reported for members of the Plumbaginaceae until recently for L. bellidifolium Durmort. The induction of somatic embryogenesis from cotyledon explants in a modified Murashige and Skoog (MS) medium was examined in four other members of this family, Limonium aureum O. Kuntze, L. latifolium O. Kuntze, L. sinuatum Mill., and Armeria maritima Willd. Induction of embryogenic callus was achieved in all the species examined on MS medium supplemented with 4.5 μm 2,4-D and 88 or 118 mm sucrose. Species of the genus Limonium responded better than A. maritima Willd. in somatic embryo induction and maturation. Somatic embryos of L. aureum O. Kuntze matured readily on MS medium supplemented with 0.93 μm kinetin and 88 mm mannitol. Chemical name used: 2,4-Dichlorophenoxy acetic acid (2,4-D).

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Somatic embryos derived from walnut (Juglans regia L.) ovule tissues were evaluated to determine whether they were of zygotic or maternal origin. Molecular markers were used to permit evaluation at an early stage, before whole plant development. Somatic embryos developed from potentially apomictic `Sunland' and `Cisco' ovule tissue isolated from bagged putatively unpollinated flowers. Phosphoglucomutase (PGM) isozyme analysis showed that all of these embryos, except one from each cultivar, carry the same zymotype as the maternal tissue. However, restriction fragment length polymorphism (RPLP) analysis combined with isozyme evaluation demonstrated that the tested embryos originated from zygotic rather than maternal tissues. This study demonstrates the application of molecular marker analyses, particularly RFLPs, evaluation of somatic embryo origin.

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