Preplant soil testing is essential for optimizing phosphorus (P) fertilization and minimizing the potential for soil P losses. Currently, there is no effective soil P extractant for calcareous soils in Florida. This study was conducted to compare Mehlich-3, ammonium bicarbonate–diethylenetriaminepentaacetic acid (AB-DTPA), and Olsen for evaluating P availability, estimating soil-test P (STP) critical levels, and calibrating P application rates for fresh-market tomato (Solanum lycopersicum L.) production in a calcareous soil. Tomatoes were grown during Winter 2014 and 2015 with P application rates of 0, 29, 49, 78, 98, and 118 kg·ha‒1 P. Water-extractable P (water-P) and dissolved reactive P (DRP) in leachate were used to determine the STP change point of leaching potential. Results showed the greatest correlation occurred between Mehlich-3 and Olsen of the three STP extractants. For Mehlich-3-P, the medium STP level (producing 75% to 90% relative yield) was predicted from 76 to 89 mg·kg‒1 and the change point was predicted at 88 or 104 mg·kg‒1 by split-line models. The P requirement was calculated from 52 to 112 kg·ha‒1 when Mehlich-3-P was rated as low level (producing 50% to 75% relative yield), which was from 42 to 76 mg·kg‒1. The multiple regression models using AB-DTPA-P and Olsen-P could not predict either the medium STP level or the practical P application rates for the low level. Consequently, based on 2 years of data, Mehlich-3 was the most effective extractant for estimating soil P availability and calibrating P rates in calcareous soils with an extremely high calcium carbonate (CaCO3) content.