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  • Author or Editor: Michelle Scheiber x
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Colchicine application successfully induced tetraploids from in vitro-cultured diploid Dieffenbachia × ‘Star Bright M-1’. Shoot clumps, each with six to eight small, undifferentiated shoot primordia, were cultured in liquid Murashige and Skoog (MS) medium and treated with colchicine at rates of 0, 250, 500, or 1000 mg·L−1 for 24 h. In vitro survival of shoot clumps significantly decreased as colchicine concentrations increased. Shoot clumps that survived were transferred to colchicine-free MS medium containing 2.0 mg·L−1 N6-isopentenyl) adenine and 0.10 mg·L−1 indole-3-acetic acid. Shoots were harvested during four subsequent subcultures and planted in a soilless substrate in a shaded greenhouse. The number of plants that survived 6 months after ex vitro planting was 690, 204, 59, and 69 for colchicine treatments at 0, 250, 500, and 1000 mg·L−1, respectively. The 332 plants from colchicine treatments along with 90 control plants (selected from 690 in the control treatment) were evaluated morphologically in a shaded greenhouse. Overall plant growth, including crown height, plant canopy, and leaf size, of colchicine-treated plants was significantly less than controls. Based on the growth data, 10, 32, 15, and 16 plants from the 0, 250, 500, and 1000 mg·L−1 colchicine rates, respectively, were selected and analyzed by flow cytometry. Flow cytometry confirmed the presence of 13 tetraploids and 29 mixoploids among the 63 colchicine-treated selections; all 10 plants from the control were diploid. A colchicine rate of 500 mg·L−1 produced a higher percentage of tetraploids (10.2%) than did the 250 (2.9%) or 1000 mg·L−1 (1.4%) rates. Subsequent comparisons showed tetraploids had significantly smaller and thicker leaves, greater specific leaf weights, and longer stomata than diploids. Tetraploids also showed increased net photosynthetic rate, decreased g S, decreased intercellular CO2 concentration, decreased transpiration rate, and increased water use efficiency. Tetraploids appeared robust and their smaller size could make them potentially more durable plants used as living specimens for interior decoration.

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The survival of shrubs planted into the landscape depends on sufficient irrigation during the establishment period. Few studies have investigated the effect of irrigation frequency on the posttransplant growth of landscape shrubs. We conducted two studies in U.S. Department of Agriculture hardiness zone 10b over a 2-year period in which we compared canopy growth index (GI), root extension to canopy spread ratio, canopy dry weight, and root dry weight of shrubs irrigated at different frequencies. In the first experiment, wild coffee (Psychotria nervosa) and ‘Lakeview’ orange jasmine (Murraya paniculata) shrubs were planted in Sept. 2004, Dec. 2004, Mar. 2005, and June 2005 and irrigated for 28 weeks after planting (WAP) every 2, 4, or 8 days with 3 L of water per irrigation event. In the second experiment, ‘Macafeeana’ copperleaf (Acalypha wilkesiana) and orange jasmine shrubs were planted in Sept. 2005, Dec. 2005, Mar. 2006, and June 2006 and irrigated for 28 WAP every 1, 2, or 4 days with 3 L of water per irrigation event. Canopy GI and root extension to canopy spread ratio were determined at 28, 52, and 104 WAP. The entire canopy and roots were harvested 52 and 104 WAP to determine dry weight. In Expt. 1, wild coffee and orange jasmine plants irrigated every 2 days had greater GI than plants irrigated every 8 days at 28 WAP, but GI was not different at 52 or 104 WAP. Canopy dry weight at 52 WAP was greater for plants irrigated every 2 days than every 8 days, but there was no difference at 104 WAP. There was no difference in wild coffee or orange jasmine root dry weight or root extension to canopy spread ratio among the irrigation frequencies. In Expt. 2, there were no differences in GI, canopy dry weight, root dry weight, or root extension to canopy spread ratio of copperleaf or orange jasmine irrigated everyday compared with plants irrigated every 2 or 4 days. From the data collected in these studies, it appears that irrigating wild coffee or orange jasmine every 8 days during the first 28 WAP limited canopy growth but not root development. However, after 52 WAP, rainfall events appeared to be sufficient to eliminate any initial effects from irrigation frequency. Our data suggest that wild coffee, orange jasmine, and copperleaf from 3-gal containers can be successfully established in the landscape when irrigated with 3 L of water every 4 days for the first 28 WAP.

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