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Michael R. Spafford and Gary J. Kling

Cercis canadensis, Eastern Redbud, is very Cercis canadensis, Eastern Redbud, is very susceptible to infection by Verticillium Wilt caused bysusceotible to infection by Verticillium Wilt caused by the common soil-borne fungi Verticillium albo-atrum and Verticillium dahliae. Little is known about the inoculum levels, the time required for natural infection to occur and how fast the pathogen travels inside the host species. One-year-old Cercis canadensis seedlings were planted in 7.6 liter (2-gallon) containers with a 1:1:2 soil/sand/perlite mix inoculated with five levels (0, 10, 100, 500, and 1000 microsclerotia/g soil) of V. dahliae prior to planting. At the end of the first growing season, half of the plants were removed from the containers, surface sterilized, dissected and root sections plated out on a Verticillium selective media. The remaining plants were grown for a second season. Infection first occurred in plants which received 100, 500 or 1000 ms/g at the end of the first season. The infection had spread at least 5 cm during the first growing season.