The applicability of β- glucuronidase and chloramphenicol acetyltransferase reporter genes to a carnation (Dianthus caryophyllus L.) transformation procedure, was analyzed. Transgenic tobacco (Nicotiana tabacum L.) plants expressing the respective reporter genes were prepared and used as the enzyme source. Carnation leaf extract strongly inhibited enzymatic activity of β- glucuronidase, but not that of chloramphenicol acetyltransferase. One or more carnation phenolic compounds, acting in a noncompetitive manner, is suggested as the cause of the observed inhibition of fluorometrically assayed β- glucuronidase activity. This inhibition was eliminated by treating the carnation leaf extract with polyvinylpolypyrrolidone.
Alexander Vainstein, Morly Fisher, and Meira Ziv
Vered Naor, Jaime Kigel, and Meira Ziv
The effect of low-temperature (LT) storage at 10 °C on dormancy relaxation and budbreak was studied in tubers of colored Zantedeschia. LT storage for 35 or 64 days enhanced budbreak by about 80 days compared to tubers stored at 20 °C. Primary bud elongation did not occur at 10 °C for 143 days, but took place after the tubers were transferred to 20 °C. Prolonged LT storage enhanced the rate of bud elongation at 20 °C. It is proposed that LT storage enhances dormancy relaxation but, at the same time, inhibits bud elongation in nondormant buds. This dual effect can serve growers in programmed production of year-round forcing of colored calla lily flowers.