Podophyllotoxin is used for the production of the anticancer drugs etoposide, etopophos, and teniposide. Currently, podophyllotoxin is extracted from the Himalayan mayapple (Podophyllum hexandrum Royle). Some junipers and other species also contain the same natural product and have been explored as a domestic source for this compound. The objective of this study was to screen junipers in the Big Horn Mountains in Wyoming for podophyllotoxin. Twenty junipers (18 accessions of Juniperus horizontalis Moench. and two accessions of J. scopulorum Sarg.) were sampled in Mar. 2012 and analyzed for podophyllotoxin. Podophyllotoxin concentration in the samples varied from 0.058% to 0.673% with five accessions having podophylloxin concentration above 0.5%. This study demonstrated wide variation of podophyllotoxin in J. horizontalis and J. scopulorum in the Big Horn Mountains. Some of the accessions had greater than 0.5% podophyllotoxin making them a feasible source for podophyllotoxin extraction.