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- Author or Editor: Mary-Howell R. Martens x
Abstract
Various procedures were compared to identify a technique that would produce clear countable chromosomes in grape (Vitis L.) root tip squashes. Harvesting roots at 1100 hr from greenhouse-grown plants, pretreating with 0.02 m 8-hydroxyquinoline at 18°C for 6 hr, fixing in Farmer's Fluid at 26° for 24 hr, hydrolyzing in 1 n HCl at 60° for 1 hr, and staining with an altered form of carbol fuchsin gave the best results.
Abstract
Pollen from 157 genotypes of grape (Vitis sp.) were scanned for the occurrence of grains ≥30 μm in diameter. Eighteen diploid genotypes produced from 3% to 36% large pollen grains. Four of these produced distinctly oval-shaped grains. Eleven tetraploid genotypes produced from 47% to 87% large pollen. Large grains in diploid genotypes may be unreduced gametes that could be used in interploid crossing to breed hybrid tetraploids.