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- Author or Editor: Marikis N. Alvarez x
Abstract
About 2000 pollinations of Pbaseolus coccineus × P. acutifolius, P. coccineus × P. vulgaris, P. vulgaris × P. acutifolius, and F2 (P. vulgaris × P. coccineus) × P. acutifolius produced more than 1500 excisable embryos, about half of which elongated and produced leaves and roots when embryo cultured on a relatively simple medium in the dark. Damaging cotyledonary buds on small embryos or removing cotyledons from larger embryos before culture enhanced germination. Transplanting germinated embryos into pots resulted in greater than 50% mortality. Hybrids tended to be intermediate to parental species in vegetative and floral characteristics but percent stainable pollen was lower. Anthers did not dehisce and percent stainable pollen was lowest in P. vulgaris × P. acutifolius and F2 (P. vulgaris × P. coccineus) × P. acutifolius hybrids. Since P. coccineus × P. acutifolius and P, coccineus × P. vulgaris hybrids were sufficiently fertile to produce advanced generations, P. coccineus may be useful as a bridge between P. vulgaris and P. acutifolius.