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Marihelen Kamp-Glass

If we want our students to engage in complex intellectual tasks to interrogate the insights of different disciplines, then let's join them in the task, modeling it and sharing the difficulties and richness of its possibilities. Interdisciplinary study is not rejection of the disciplines. It is firmly rooted in them, but offers a corrective to the dominance of disciplinary ways of knowing and speculation. We need the depth and focus of disciplinary ways of knowing, but we also need interdisciplinarity to broaden the context and establish links to other ways of constructing knowledge. It is this dialogue between analysis and synthesis that provides the creative tension from which we will all benefit in a world in which crossing intellectual boundaries is increasingly the norm.

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Marihelen Kamp-Glass*

Students of today have been raised on 20 second sound bytes, video games, multiple choice and true false exams, extra credit, and over extended parents It is difficult to engage most of them in a two minute conservations. They have not been taught the skill of cognitive thinking. We as professors need to help them reach that higher level of oral and written communitation. This can be done through adding discussion in the class room, on a field trip, or in an e-College or Blackboard venue. This presentation is abou how good discussions must be prepared in advance, how to lead a purposefully discussion and other way to promote their cognitives skills.

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Guochen Yang and Marihelen Kamp-Glass

Multiple shoots were produced directly from each explant. BA, zeatin, or TDZ were evaluated on callus initiation, development, and shoot organogenesis. Callus production was promoted when BA or zeatin was added in culture medium. However, no shoots were produced from such calli. Multiple shoots were produced directly through shoot organogenesis from each seed explant when TDZ was added to culture medium. As many as 30 to 40 shoots were produced per seed explant in about 7 weeks from culture initiation. Different alfalfa cultivars were also tested. The established multiple shoot production protocol provides an efficient way to produce transgenic alfalfa plants, thus could significantly advance alfalfa genetic transformation.

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Guochen Yang and Marihelen Kamp-Glass

The success of genetic transformation depends on the efficiency and rehability of in vitro shoot regeneration. This research was pursued to investigate how different plant growth regulators influence alfalfa callus initiation and development, thus to establish a foundation for further development of an efficient shoot organogenesis protocol for the genetic transformation system. BA, zeatin, and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) were evaluated for callus initiation and development. BA at 1 or 5 mg·liter–1, or zeatin at 5 mg·liter–1 promoted callus regeneration and further development toward shoot organogenesis. However, 2,4,5-T at 1 mg·liter–1 enhanced only callus production. These results can and will be used for further development of a shoot regeneration protocol to assist alfalfa genetic transformation.

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Guochen Yang and Marihelen Kamp-Glass

Exochorda racemosa is an ornamental shrub with white flowers that is spiraea-like, deciduous, and hardy. The buds resemble pearls. Normally it is propagated by seeds, layers, and cuttings of softwood. However, it is a slow process that takes a few years to produce a reasonable size plant for the demanding market. Our objective was to establish a successful in vitro culture and to rapidly multiply this ornamental species. Softwood explant materials were collected from a local nursery and were disinfested with 15% bleach solution and rinsed three times with sterile distilled and deionized water. In vitro cultures were established and maintained in woody plant medium (WPM) supplemented with BA at 0.1 mg·L-1, 3% sucrose, and 0.7% agar with the pH adjusted to 5.8. Then shoots were transferred to the multiplication medium containing BA, CPPU, or thidiazuron (TDZ) at various concentrations. Preliminary results show that explants cultured on medium containing TDZ produced the best shoot proliferation.

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Guochen Yang and Marihelen Kamp-Glass

An efficient and reliable protocol of in vitro shoot regeneration must be first established to have a successful genetic transformation. As a member of legume family, alfalfa is very difficult for direct shoot regeneration. There is no published information on direct shoot organogenesis, although success has been well documented on embryogenesis, which must go through callus stage. Different plant growth regulators at various concentrations were evaluated for callus initiation, development, and direct shoot regeneration. Multiple shoots were produced directly from each individual explant. This will provide an efficient means for production of transgenic alfalfa plants. Therefore, genetic transformation of Medicago germplasm will be significantly expedited.

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Karen Neil, Carl Niedziela, and Marihelen Kamp-Glass

A study was conducted with pansies (Viola wittrockiana) on a tobacco float bed system to determine if plugs could successfully be grown into transplants and to determine nutrient levels that wouldprovide the best transplants. Transplants were grown in a soilless media in 72-cell polystyrene float trays, floating on four different nutrient concentrations: 25, 50, 100, or 200 ppm. Length of time needed to produce regular-size transplants is reduced by half. Only 3 weeks are needed using the floating bed system, while 6 to 9 weeks are needed for the conventional method. Transplants displayed vigorous growth with normal morphology. Plant height and weight are significantly enhanced by increasing the nutrient concentration.

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Janice Smith, Charles Raczkowski, and Marihelen Kamp-Glass

Crop canopy cover data is used to study canopy structure and crop growth analysis. This study was conducted to determine the easiest and most reliable method of calculating crop canopy cover. Using Decagon Sunfleck Ceptometer was compared with the traditional method (tape measure) of retrieving crop canopy cover data. Data was collected on silage corn (Zea mays) and soybeans (Glycine max L.). The method of collecting data using the ceptometer was simple and quick compared to the traditional method. The ceptometer, even with human variability, was found to be ≈99% accurate. The traditional method was found to have >10% variability. The ceptometer is a much quicker and more reliable tool to use. It appears to decrease the variability in the collection of crop canopy cover data.

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Monica Haddix, Marihelen Kamp-Glass, and Charles Raczkowski

Encyclia is a genus of orchids with four members native to Florida that are Endangered or commercially exploited. If a commercial orchid tissue culture media was found that could be used to propagate Encyclia, the resulting plants could be introduced into protected habitats to increase the wild populations or could be grown commercially to lessen the demand for collected plants. Six month Encyclia tampensis (Lindl.) seedlings were grown in Orchid Maintenance/Replate Medium w/Banana and Charcoal with 6 g·L–1 agar (C), ½ strength Orchid Multiplication Medium with 6 g·L–1 agar and 10 g·L–1 charcoal (OM), Knudson C Orchid Medium – Morel Modification with 6 g·L–1 agar and 10 g·L–1 charcoal (K), or Vacin and Went Modified Orchid Medium w/agar and sucrose with 10 g·L–1 charcoal (VW) for seven weeks. Plants grown in OM and VW media both produced more shoots and roots (7.56 leaves, 1.92 shoots and 6.67 leaves, 1.76 shoots respectively) than the control medium (5.87 leaves, 1.70 shoots) with OM producing the highest averages. Plants grown in the K medium averaged significantly less shoots and leaves than the other three media (4.94 leaves, 1.35 shoots).

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Janice Smith and Marihelen Kamp-Glass Dr.

Effect of gibberellin (GA) concentration on Chrysanthemum (Pink Adorn, White Adorn, Daisy Royal, and Target) were investigated. The controlled and noncontrolled were observed. GA 1.5 was applied to the noncontrolled garden mums at 1, 2, 5, and blank ppm concentrations. The controlled and noncontrolled was evaluated after one week. Normal growth rate on controlled. Growth rate on noncontrolled at 1, 2, and 5ppm was evidently accelerated on all varieties, while GA blank decreased the growth rate of all varieties. GA at 1, 2, and 5ppm affected the Target variety more than any other variety.