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  • Author or Editor: Marihelen Kamp-Glass x
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If we want our students to engage in complex intellectual tasks to interrogate the insights of different disciplines, then let's join them in the task, modeling it and sharing the difficulties and richness of its possibilities. Interdisciplinary study is not rejection of the disciplines. It is firmly rooted in them, but offers a corrective to the dominance of disciplinary ways of knowing and speculation. We need the depth and focus of disciplinary ways of knowing, but we also need interdisciplinarity to broaden the context and establish links to other ways of constructing knowledge. It is this dialogue between analysis and synthesis that provides the creative tension from which we will all benefit in a world in which crossing intellectual boundaries is increasingly the norm.

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Students of today have been raised on 20 second sound bytes, video games, multiple choice and true false exams, extra credit, and over extended parents It is difficult to engage most of them in a two minute conservations. They have not been taught the skill of cognitive thinking. We as professors need to help them reach that higher level of oral and written communitation. This can be done through adding discussion in the class room, on a field trip, or in an e-College or Blackboard venue. This presentation is abou how good discussions must be prepared in advance, how to lead a purposefully discussion and other way to promote their cognitives skills.

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Multiple shoots were produced directly from each explant. BA, zeatin, or TDZ were evaluated on callus initiation, development, and shoot organogenesis. Callus production was promoted when BA or zeatin was added in culture medium. However, no shoots were produced from such calli. Multiple shoots were produced directly through shoot organogenesis from each seed explant when TDZ was added to culture medium. As many as 30 to 40 shoots were produced per seed explant in about 7 weeks from culture initiation. Different alfalfa cultivars were also tested. The established multiple shoot production protocol provides an efficient way to produce transgenic alfalfa plants, thus could significantly advance alfalfa genetic transformation.

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The effectiveness of antitranspirant type and concentration on the leaf water relations of Saliva splendens F. `Firebird and Petunia × hybrida Juss. `Comanche'. Two film-forming antitranspirants, Cloud Cover and Folicote, were tested at three different concentrations in two different environments. The leaf water potential, stomatal conductance, and relative water content were evaluated. Transpiration per unit vapor pressure deficit and stomatal conductance for both crops decrease slightly but there was no trend with respect to the film type, environment or concentration rate. The leaf water potentials and relative water content did not show significant difference after antitranspirant application. In order for antitranspirant application to be of benefit to the growth of herbaceous plants, a more durable coating that remains semipermeable would have to be utilized.

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The effects of ORC and different commercial plant fertilizers on Marigolds, Salvias, and Begonias were determined by height, flower number, fresh weight, dry weight, root length and tissue analysis. The treatments included controls, regular fertigations, and an over fertigation schedule. The experiment was set in a randomized block design with 5 reps per treatment which included 9 ORC and commercial plant fertilizer combinations, 4 commercial plant fertilizers, and controls. The plants with the ORC were more vigorous, had more flowers, and better overall color than the controls.

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Effect of gibberellin (GA) concentration on Chrysanthemum (Pink Adorn, White Adorn, Daisy Royal, and Target) were investigated. The controlled and noncontrolled were observed. GA 1.5 was applied to the noncontrolled garden mums at 1, 2, 5, and blank ppm concentrations. The controlled and noncontrolled was evaluated after one week. Normal growth rate on controlled. Growth rate on noncontrolled at 1, 2, and 5ppm was evidently accelerated on all varieties, while GA blank decreased the growth rate of all varieties. GA at 1, 2, and 5ppm affected the Target variety more than any other variety.

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Exochorda racemosa is an ornamental shrub with white flowers that is spiraea-like, deciduous, and hardy. The buds resemble pearls. Normally it is propagated by seeds, layers, and cuttings of softwood. However, it is a slow process that takes a few years to produce a reasonable size plant for the demanding market. Our objective was to establish a successful in vitro culture and to rapidly multiply this ornamental species. Softwood explant materials were collected from a local nursery and were disinfested with 15% bleach solution and rinsed three times with sterile distilled and deionized water. In vitro cultures were established and maintained in woody plant medium (WPM) supplemented with BA at 0.1 mg·L-1, 3% sucrose, and 0.7% agar with the pH adjusted to 5.8. Then shoots were transferred to the multiplication medium containing BA, CPPU, or thidiazuron (TDZ) at various concentrations. Preliminary results show that explants cultured on medium containing TDZ produced the best shoot proliferation.

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The success of genetic transformation depends on the efficiency and rehability of in vitro shoot regeneration. This research was pursued to investigate how different plant growth regulators influence alfalfa callus initiation and development, thus to establish a foundation for further development of an efficient shoot organogenesis protocol for the genetic transformation system. BA, zeatin, and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) were evaluated for callus initiation and development. BA at 1 or 5 mg·liter–1, or zeatin at 5 mg·liter–1 promoted callus regeneration and further development toward shoot organogenesis. However, 2,4,5-T at 1 mg·liter–1 enhanced only callus production. These results can and will be used for further development of a shoot regeneration protocol to assist alfalfa genetic transformation.

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An efficient and reliable protocol of in vitro shoot regeneration must be first established to have a successful genetic transformation. As a member of legume family, alfalfa is very difficult for direct shoot regeneration. There is no published information on direct shoot organogenesis, although success has been well documented on embryogenesis, which must go through callus stage. Different plant growth regulators at various concentrations were evaluated for callus initiation, development, and direct shoot regeneration. Multiple shoots were produced directly from each individual explant. This will provide an efficient means for production of transgenic alfalfa plants. Therefore, genetic transformation of Medicago germplasm will be significantly expedited.

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Venus fly trap, Dionaea muscipula Ellis, leaf sections were surfaced sterilized under aseptic conditions. The leaf sections were cultured in reduced strength Muashige and Skoog growth medium supplemented with 2,25 mg/l 6-Benzylaminopurine and 1.0-2.0 mg/l Kinetin. The various levels of cytokinin were used to differentiate and enhance callus formation.

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