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  • Author or Editor: Maria Chrzastek x
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Heterozygous multiple marker genetic stocks were synthesized by crossing three multiple genetic marker stocks to a common inbred parent PU812. The four parents and 3 F1's were cultured to obtain regenerants from leaf discs. Fifty four regenerants were derived from 3 F1's and 12 from the 4 parents, Among the regenerants, 16 plants were identified as tetraploid (24.2%); low fertility was usually associated with tetraploidy, however there were a few exceptions.

Selfed seeds, identified by cluster number, were harvested from sexual F1's and R0 plants for F2 progeny tests for the known marker genes. While there were no abnormal segregations for marker genes in the sexual progenies, 13 of 46 progenies from tissue culture derived regenerants showed significant deviations from expected normal segregations for a number of markers. Two of the abnormal progenies were identified as tetraploids by root-tip examinations; segregation ratios fit duplex random chromatid segregation for gene a on chromosome 11 and random chromosome segregation for gene c on chromosome 6. The cams of abnormal segregations in other progenies remain unknown. Results suggest that unknown genetic events arising during tissue culture may distort segregations for marker genes in the subsequent sexual progeny of tissue culture regenerants.

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