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- Author or Editor: Margaret Worthington x
Rooting hardwood cuttings from muscadine (Vitis rotundifolia Michx. syn. Muscadinia rotundifolia) vines has traditionally been considered an exceptionally difficult task. Many previous studies observed almost no root formation, leading to a general consensus that muscadines should either be propagated by softwood cuttings or vegetative layering. However, the University of Arkansas System Division of Agriculture Fruit Breeding Program has been using a hardwood rooting protocol for muscadines with moderate success for the past 10 years. The application of this protocol to meet the modest propagation needs of the breeding program has significantly shortened the time required to advance selections. The goal of this research was to more adequately describe the factors affecting the rooting ability of hardwood muscadine cuttings. This research investigated the effects of cultivar, bottom heat, cold storage, vineyard location, and cutting collection date on the outcome of muscadine hardwood cuttings. The study was conducted during the dormant seasons of 2019–20 and 2020–21, and an overall rooting percentage of 16% was observed. There were multiple higher-order interactions affecting rooting efficacy. Cuttings taken in November generally rooted at higher rates, although interactions with vineyard location and cultivar played a significant role in those results. The Ocilla, GA, location performed exceptionally well in November with rooting percentages greater than 40%. The effects of supplying bottom heat and/or a cold storage treatment on rooting success declined as the dormant season progressed. Other variables such as increased cutting length and diameter were associated with increased rooting success. A second statistical analysis using only data from November showed that when cuttings were not given a cold storage treatment that rooting percentages were greater than 27%. Ultimately, this research shows that institutions with modest muscadine propagation needs can successfully propagate plants from hardwood cuttings.
Understanding how human perception is related to physicochemical attributes strengthens identification of ripeness and marketability parameters for peaches and nectarines [Prunus persica (L.) Batsch]. Six peach and nectarine cultivars (Amoore Sweet, Bowden, Effie, Loring, Souvenirs, and White River) and three advanced breeding selections (A-827, A-850, and A-865) were harvested from trees grown at the University of Arkansas System Division of Agriculture Fruit Breeding Program in Clarksville, AR. Physical and chemical characteristics of the genotypes at harvest were as follows: fruit weight of 134.4 to 330.2 g, firmness of 7.8 to 35.8 N, soluble solids of 7.5% to 14.7%, pH of 3.3 to 4.8, titratable acidity of 0.2% to 1.1%, total sugars of 1.7 to 10.4 g/100 g, and total organic acids of 0.1 to 0.9 g/100 g. Overall, A-865 had the lowest fruit weight (134.0 g) and pH (3.3), and the highest firmness (35.8 N), soluble solids (14.7%), titratable acidity (1.1%), total sugars (10.4 g/100 g), and total organic acids (0.8 g/100 g). ‘White River’ had the largest fruit (330.2 g) and pit (11.06 g). A-850 (63.6) had the highest soluble solids/titratable acidity ratio, and ‘Bowden’ (12.7) had the lowest. A trained descriptive sensory panel (n = 10) was used to create a lexicon for Arkansas-grown fresh-market peaches and nectarines. The panel evaluated the fruit for aroma (n = 4), external appearance (n = 8), internal appearance and pit attributes (n = 6), basic tastes (n = 3), aromatics while eating fruit (n = 5), feeling factors (n = 2), and texture (n = 6). Principal component analysis explained 63.4% of the data variance attributed to texture and acidity. Of all of the physicochemical attributes, firmness had the most significant correlations with the descriptive sensory attributes, followed by fruit weight. Firmness was negatively correlated (r = −0.70 to 0.81) to fruit size, fuzziness, amount of bruises on the flesh, pit size, and moisture release, and positively correlated (r = 0.68–0.84) to sourness, green/unripe aromatics, flesh hardness, flesh crispness, and fibrousness between the teeth. Fruit weight was positively correlated (r = 0.67–0.75) to fruit and pit size, overripe aromatics, and moisture release. Significant correlations between descriptive sensory appearance, basic tastes, aromatics, and texture attributes with physicochemical attributes provide an indication of ripeness and marketability parameters for peaches and nectarines. These descriptive attributes are quality factors that impact consumer purchases and perception of fresh-market peaches and nectarines.
Breeding and release of new fresh-market blackberries (Rubus subgenus Rubus Watson) is vital for competitive markets to address evolving changes and production challenges. Physical, composition, and sensory attributes of six University of Arkansas (UA) System Division of Agriculture blackberry cultivars (Caddo, Natchez, Osage, Ouachita, Ponca, and Prime-Ark® Traveler) were evaluated to identify marketable attributes. The consumer sensory study (n = 81) had two elements: a visual evaluation of displayed blackberries and an appearance, tasting, and firmness evaluation of the six cultivars using a 9-point verbal hedonic liking scale and a 5-point just about right (JAR) scale. Consumers preferred large blackberries when presented with individual berries of varying sizes and clamshells filled with equal weights of small or large blackberries. The largest of the six cultivars, Natchez and Caddo, were scored favorably for size and shape. Consumers also preferred clamshells with little to no red drupelet reversion, a postharvest disorder where black drupelets on the blackberry turn red during or after cold storage. Consumers did not detect differences in the appearance or firmness of the cultivars and rated the firmness of all cultivars favorably on the JAR scale. The physical and composition attributes of the six cultivars were within commercially acceptable ranges (soluble solids = 9% to 10%, pH = 3.1–3.8, titratable acidity = 0.6% to 1.4%, and berry weight = 6–10 g). ‘Ponca’, ‘Osage’, ‘Caddo’, and ‘Natchez’ were all rated highly for sweetness, sourness, overall flavor, and overall impression. ‘Ponca’ was rated high for sweetness, overall flavor, and overall impression and had 10.4% soluble solids, 0.82% titratable acidity, and a 12.8 soluble solids/titratable acidity ratio. The identification of these marketability attributes of fresh-market blackberries will provide information to advance breeding efforts for fruit with commercial potential.
Red drupelet reversion (RDR) is a postharvest disorder of blackberries (Rubus L. subgenus Rubus Watson) in which fully black drupelets revert to red after harvest. This disorder can negatively impact consumer perception of fresh-market blackberries. The cause of RDR is hypothesized to be related to intracellular damage sustained because of mechanical and environmental stress during and after harvest. Cultivars differ in susceptibility to this disorder; and cultural factors, including nitrogen rate, harvest and shipping practices, and climate during harvest, influence RDR severity. In this 2-year study, seven genotypes (cultivars and advanced selections) developed in the University of Arkansas System Division of Agriculture (UA) blackberry breeding program, with a range of fruit textures, were evaluated to determine whether firmness was correlated with RDR. In addition, fruit was harvested at four different times (7:00 am, 10:00 am, 1:00 pm, and 4:00 pm) to investigate whether harvest time influences RDR. All seven genotypes were harvested at the four times on two harvest dates per year and evaluated for RDR and firmness after 1 week of cold storage (5 °C). Fruit harvested early in the day had less RDR, with 7:00 am harvests having the least RDR in both years. Significant genotypic differences in RDR and fruit firmness were found in each year. Firmness was negatively correlated with RDR in 2018 and 2019. These results indicate that growers may be able to reduce the prevalence of RDR by choosing cultivars with firm fruit texture and harvesting early in the day.
Understanding how consumer perception is related to physiochemical attributes assists in the identification of harvest and marketability parameters for muscadine grapes (Vitis rotundifolia Michx.). Three muscadine cultivars (Ison, Nesbitt, and Summit) and three advanced breeding selections (AM-9, AM-74, and AM-83) were harvested from vines at the University of Arkansas System Division of Agriculture Fruit Research Station in Clarksville, AR. The physiochemical (physical and composition) and sensory attributes (descriptive) of the genotypes were evaluated at harvest. Significant differences between genotypes were observed for berry weight (9.25–14.38 g), soluble solids (12.73% to 15.40%), pH (2.88–3.33), titratable acidity (0.54% to 1.01%), soluble solids/titratable acidity ratio (13.12–28.49), skin firmness [0.85–1.48 Newtons/millimeters (N·mm−1)], and flesh firmness (0.89–2.14 N). Total sugars (6.17–9.75 g/100 g) and total organic acid (0.50–0.84 g/100 g) levels were not significantly different for these genotypes. A trained descriptive sensory panel (n = 8) evaluated the fruit attributes for aroma (n = 9), external appearance (n = 7), internal appearance (n = 3), basic tastes (n = 3), aromatics (n = 10), feeling factors (n = 2), and texture (n = 7). The descriptive sensory panel detected differences among genotypes for external appearance, internal appearance, and basic taste attributes, more specifically with desirable attributes rather than unfavorable. However, the panelists found no differences among genotypes for texture attributes. Of the physiochemical attributes, total sugars had the most significant correlations with the descriptive sensory attributes, followed by soluble solids/titratable acidity ratio. Total sugars were correlated to 12 attributes (three aromas, two exterior appearances, two basic tastes, four aromatics, and one feeling factor) and soluble solids/titratable acidity was correlated to five attributes (one aroma, one basic taste, two aromatics, and one feeling factor). A lexicon of terms for descriptive sensory attributes for fresh-market muscadine grapes was established. This lexicon can be used for other research and breeding efforts, as well as establishing the relationship between the physiochemical and descriptive sensory attributes.
Confirming parentage and clonal identity is an important aspect of breeding and managing germplasm collections of clonally propagated, outcrossing crops, like blackberry (Rubus subgenus Rubus). DNA fingerprinting sets are used to identify off-cross progeny and confirm clonal identity. Previously, a six-simple sequence repeat (6-SSR) fingerprinting set was developed for blackberry using a small number of samples. The usefulness of the 6-SSR fingerprinting set for pedigree confirmation had not been evaluated. Therefore, it was used in this study to validate parentage for 6 and 12 biparental populations from the University of Arkansas (UA) and US Department of Agriculture Agricultural Research Service (USDA-ARS), Horticultural Crops Research Unit (HCRU) breeding programs, respectively. Twenty-seven of the 489 individuals in these breeding populations were identified as off-cross. The 6-SSR fingerprinting set was sufficient for parentage confirmation; however, a total of 61 plants distributed across 28 sets of genotypes could not be distinguished from each other. An 8-SSR fingerprinting set with improved resolution was subsequently developed and used to evaluate 177 Rubus accessions from the USDA-ARS National Clonal Germplasm Repository, UA, and USDA-ARS HCRU programs. The 8-SSR fingerprinting set distinguished all samples expected to have unique genotypes and identified differing DNA fingerprints for two sets of accessions suspected to have identical fingerprints. Cluster analysis grouped the accessions from the eastern and western US breeding programs based on geography and descent. Future work will focus on establishing a database of DNA fingerprints for germplasm identification and for determining pedigree relationships between blackberry accessions.