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This study was carried out to investigate the distribution, morphological and ecological characteristics, cross fertility and peroxidase banding pattern in floral buds of wild Prunus yedoensis var. nudiflora and its putative parents, P. subhirtella and P. donarium growing at Haenam districts in Korea. P. yedoensis was distributed 200-250 m elevation and showed extensive variations in sterility and morphological variations. The morphological characteristics of P. yedoensis are judged to be intermediate between P. subhirtella and P. donarium. P. subhirtella and P. donarium were naturally found 150-350 m and 150-900 m elevation. Flowering date of p. subhirtella, P. donarium and p. Yedoensis were early April, mid-to-late April and around April 8, respectively. The floral morphology of p. yedoensis showed greater variations than that of p. subhirtella. It is proved that p. yedoensis is an interspecific hybrid between P. subhirtella and P. donarium considering the intermediate characteristics of flower, leaf and peroxidase banding pattern of the floral buds by isoelectric focusing electrophoresis.
From one week through 7 weeks after artificial pollination, immature ovules of yooza(Citrus junos Sieb. et Tanaka) were excised and cultured in vitro on MT media. Even though there was only a little difference in percentage of somatic embryo formation depending upon the time of excision, immature ovules of 4-week-old showed the highest ratio of somatic embryo formation without callus outgrowth. Various growth regulators or other stimulators were added to the MT media to increase the somatic embryogenesis, In general, BAP was more effective than 2,4-D for somatic embryo formation and the combinations of 0.01mg/l 2,4-D and 0,01 or 0.1mg/l BAP were particularly effective in stimulating somatic embryo formation. When 500mg/l malt extract was added to the medium, the percentage of somatic embryo formation increased reaching as high as 86.7%. Plant regeneration from somatic embryos reached to 66.7% on the medium containing 1.0mg/l zeatin. Isozyme banding patterns were also analyzed to confirm the variations of characteristics of the plantlets derived from direct somatic embryos.