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  • Author or Editor: Mahmoud A. Hassan x
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An efficient protocol for plantlet regeneration using petioles and internodes of two blackberry cultivars has been developed for use in genetic transformation. Maximum shoot regeneration was induced on MS medium supplemented with 5 μM Thidiazuron (TDZ) and 0.5 μM IBA. Preconditioning the source shoots on 0.5 μM TDZ containing MS medium did increase the number of regenerated shoots/explant, but did not affect the regeneration percentage. The antibiotic, kanamycin, significantly reduced the growth and regeneration of explants at 10 mg/L or higher. In contrast, cefatoxime at 100-500 mg/L increased explant growth and percentage regeneration.

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Nigellia sativa L. plants were fertilized with different rates of NPK fertilizers and sprayed with the growth regulators BL-2142 at 0, 250, 500, and 1000 ppm, CCC at 0, 500, 1000, and 1500 ppm and Multiprop at 0. 12.5, 25, and 50 ppm.

The results indicated that both of NPK fertilization and growth regulator treatments enhanced the plant growth in terms of stem diameter, branch number and herb dry weight. Also, these treatments caused early flowering, increased fruit number and seed yield compared to the control plants.

The interaction between NPK fertilization and growth regulators had a synergistic effect. The highest seed yield was obtained when the plants received 200, 100, and 25 kg/feddan (feddan = 4200 sqm) of urea, calcium superphosphate and potassium sulphate, respectively and sprayed with CCC at 500 ppm.

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Nigellia sativa L. plants were fertilized with different rates of NPK fertilizers in combination with the growth regulators, BL-2142, CCC and Multiprop sprayed at varied concentrations.

Fertilization and growth regulators increased the volatile and fixed oil content in the seeds. The photosynthetic pigments in the leaves, the reducing sugar and the total carbohydrate contents, N, P, and K uptake in the herb were also increased.

The interaction between fertilization and growth regulators had a synergistic effect on increasing the volatile and fixed oil percentage and yield, the photosynthetic pigments, N, P, K uptake. The highest volatile oil yield was found when the plants received 100, 200 and 50 kg/feddan (4,200 sqm) of urea, calcium superphosphate and potassium sulphate, respectively and sprayed with 500 ppm BL-2142, 1000 ppm CCC or 12.5 pm Multiprop. The volatile oil, fixed oil yield and seed yield were highly and significantly correlated with each other.

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Red radish is a nutritious root vegetable crop that has a short production cycle. Water deficit limits plant productivity, affecting its quantity and quality. Compost amendment offers a potential solution to mitigate water deficit effects. This study assessed the impact of compost manure rates (0%, 50%, 75%, and 100%) and irrigation treatments (40%, 60%, 80%, and 100% of evapotranspiration) on ‘Crimson Giant’ red radish production. Significant differences in growth and quality were observed among these treatments. Compost rates of 75% and 100% improved leaf gas exchange, plant growth (leaf count, fresh weight, dry weight, and area; stem length), root development, total yield (root fresh weight, dry weight, diameter, and length), and root quality (vitamin C and total soluble solid and titratable acidity). The 100% compost and 100% irrigation combination achieved the highest yields. Under water deficit, applying 75% or 100% compost with 80% irrigation conserved 20% of water while maintaining radish output. Overall, compost amendment effectively enhanced red radish growth and production under water deficit.

Open Access