Chloroplast DNA polymorphisms were obtained using a combination of RFLP analysis of total cpDNA and a combination of PCR amplification restriction of a 3.2-kb region of cpDNA described previously. Nuclear genome analysis was conducted using 21 RFLP probes that revealed 192 alleles. Parsimony analysis of nuclear DNA places J. cinerea between J. regia and other Asian species, while Wagner-Distance analysis places all North American species between them. cpDNA parsimony also placed the North American species between J. regia and remaining Asian species, but did not resolve the placement of J. cinerea, because all North American species were monomorphic. Both analyses support an ancient origin for J. regia and multiple migration events between North America and Asia.
Dan E. Parfitt, M.L. Badenes and R.G. Fjellstrom
F. Sanz-Cortés, M.L. Badenes, S. Paz, A. Íñiguez and G. Llácer
Forty olive (Olea europaea L.) cultivars from Valencia, Spain, were screened using random amplified-polymorphic DNA (RAPD) markers. Eighteen selected decamer primers produced 34 reproducible amplification fragments that were then used as polymorphic markers. The resulting combinations of these RAPD markers were used to discriminate 40 cultivars. Results were analyzed for similarity among cultivars and the relatedness of polymorphisms obtained between cultivars agreed with previous results using isozymes. Unweighted pair group method cluster analysis of their similarity values revealed two main groups divided according to geographic origin within Valencia. A third group, which included two Spanish cultivars from regions outside of Valencia, was clustered separately from the Valencian cultivars. RAPD technology proved useful in discriminating closely related cultivars. There was no apparent clustering of cultivars by fruit size or other morphological traits.