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  • Author or Editor: M.C. Goffinet x
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The histology of external CO2 injury of the skin of `Empire' apples and postharvest factors affecting occurrence of injury were investigated. Injury was greater in a 5% CO2/2% O2 atmosphere than in 2% CO2/2% O2, but incidence was affected by orchard source. Susceptibility to injury was highest during the first 4 weeks of storage, while a postharvest treatment with diphenylamine prevented the disorder. Ethanol reduced injury, but ascorbic acid increased incidence of the disorder. Keeping fruit in air cold storage for 10 days before application of CO2 markedly reduced incidence of CO2 injury. Histological studies showed that external CO2 injury begins at the hypodermis—cortex boundary and spreads outward into the upper hypodermis and inward into outer cortex cells, although the cuticle and epidermis appear unaffected and unbroken. Radial walls of affected cells collapse and become pleated, so that the skin surface sinks below nearby normal regions. Other cellular events include loss of cytoplasmic integrity, coagulation of the protoplast, loss of organelle structure, and cell wall separation. Nondigested starch can be found in cells of affected fruit at the hypodermis—cortex boundary. We conclude that several factors affect fruit susceptibility to CO2 injury, including orchard, antioxidant treatment, and delays before application of CO2.

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During imbibition, water always follows the same pattern when entering the seed testa in semihard seeds (SHS) of snap bean (Phaseolus vulgaris L.). Water first enters the raphe and the chalazal region of the testa (R-CT), then migrates circumferentially along the midline of the seed, leaving the lateral faces the last to be fully imbibed. The R-CT region is the main site of primary uptake of both water vapor and liquid water by SHS. The hilum, micropyle, and strophiole play only a minor role in water uptake in SHS. In comparison to the readily permeable seeds of ‘Bush Blue Lake 47’, SHS have more total phenols in the osteosclereid cells and more pectic substances in the palisade cells of the CT. The presence of these compounds may account for the impermeable nature of SHS. Measurements made of palisade cell length and width in the R-CT region revealed that cell length increased and width decreased in the chalazal testa region (CT) as seed moisture content increased from 6% to 12%. It is proposed that semihardening of bean seeds is mainly a result of the reversible physical changes in the length and width of the palisade cells in the R-CT region. Seeds imbibe at high moisture content (12%) because the palisade cells have stretched, which allows water uptake. Seeds are impermeable at low moisture content (6%) because the palisade cells change in size and form a physical barrier to water movement.

Open Access

Northeastern U.S. grape growers have become more knowledgeable about many aspects of grape production, including pruning and training, canopy management, nutritional recommendations, pest and disease management strategies, vineyard floor management, etc. Important to all these aspects is a firm understanding of vine structure and development. Yet, there is no current publication on vine growth and development that growers and researchers can consult to gain an understanding of the organs, tissues, and developmental processes that contribute to growth and production of quality vines in the northeastern U.S. climate. A concerted effort is underway to secure enough information on how vines are constructed, grow, and develop in the northeast so that a publication useful to a wide audience can be produced. Our objective is to consolidate information already on hand that can help explain the internal and external structures of grapevines that are pertinent to the needs of northeast growers, to add information that is lacking by collecting and examining vine parts, and to work toward integrating vine structure with vine physiology and viticultural practices. Over the past decade, organs of various native American, French hybrid, and vinifera varieties have been collected from vineyards at Cornell's experiment stations and from growers' vineyards in the Finger Lakes and Lake Erie regions. Much quantitative data on vine development have been collected and interpreted. Lab work has included dissections of organs, histological and microscopic examination, microphotography, and the production of interpretive diagrams and charts. A list of the subject matter and examples of visual materials will be presented.

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