Strawberry flowering habit can be classified as either day-neutral (DN) or short-day (SD), depending on whether plants are insensitive or sensitive to photoperiod, respectively. Short-day (SD) cultivars produce mature fruit for just a few weeks in early summer. New floral initiation does not commence until triggered by the combination of short daylength and low temperature in the fall. Day-neutral (DN) cultivars do not require particular daylength conditions to initiate flowering, and so continue to produce flowers and mature fruit into late summer and early fall. We are using a map-based approach to characterize the genetic determinants of flowering habit in strawberry at both the diploid and octoploid levels. A recessive gene conferring DN flowering habit has been identified, and its position determined with respect to molecular markers on the Fragaria vesca genetic linkage map. We are using the technique of bulked segregant analysis (BSA) in an effort to find random amplified polymorphic DNA (RAPD) markers linked to a putative dominant gene conferring the DN habit in the octoploid, cultivated strawberry, F. ánanassa.