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J.M. Vogel, A. Rafalski, M. Morgante, G. Taramino, W. Powell, M. Hanafey and S.V. Tingey

DNA-based diagnostics are now well-established as a means to assay diversity at the locus, chromosome, and whole-genome levels. As technology has advanced, DNA sequence-based assays have become easier to use, more efficient at screening for nucleotide sequence-based polymorphisms, and available to a wider cross-section of the research community. A review of the use of molecular markers in several different areas of genetics and plant breeding will be presented, as well as a discussion about their advantages and limitations. Recent advances in several areas of technology development and laboratory automation will also be presented, including a summary of direct comparison of different DNA marker systems against a common set of soybean cultivars.

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J.M. Vogel, A. Rafalski, W. Powell, M. Morgante, C. Andre, M. Hanafey and S.V. Tingey