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Abstract
Investigation of optimum conditions for extraction, titrimetric assay and activity of avocado (Persea americana Mill cvs. Fuerte, Hass) pectinmethylesterase (PME) showed maximum extraction was obtained from lyophilized mesocarp using 0.4 m NaCl. Best assay conditions required a substrate of 0.5% pectin in 0.1 m NaCl. The enzyme was released from the tissue by salt but not by nonionic detergents. The Arrhenius plot between 3 and 32°C was a straight line which indicates no involvement with membrane lipid. Incubation with air or ethylene did not affect the enzyme. PME activity declined rapidly as ripening was initiated and reached a minimum shortly before the peak in respiration and ethylene production.
Abstract
Applications of ethylene have been shown to be effective in removing astringency from fruits of persimmon (Diospyros kaki L.f.) (1). (2-Chloroethyl)phosphonic acid (ethephon) 4 , an ethylene generator, was applied to uniform, firm, and well colored ‘Taubate’ persimmon fruits the day after harvest in concn of 0, 250, 500 and 1000 ppm by immersion for 1 hr (2) to determine its effect on fruit astringency. The fruits were maintained at room temp.
Abstract
Squash (Cucurbita pepo), cucumber (Cucumis sativus), muskmelon (Cucumis melo) and corn (Zea mays.) plants were grown in sand culture with B as the variable. The plants were harvested when they showed a definite gradation of B toxicity symptoms from severe to none. Mature blades were analyzed for total B.
A significant decrease in top growth took place in corn and cucumber for culture solutions with B concentrations greater than 2 ppm; in squash and muskmelon, greater than 4 ppm. A 50% decrease in top growth took place in solutions with 6, 12, 12 and 16 ppm of B for cucumber, squash, muskmelon and corn, respectively, with cucumber the most sensitive and corn the least sensitive to B supply. The critical concentrations for B toxicity in mature blades at the onset of decreased top growth are 100, 400, 800 and 1,000 ppm, dry basis, for corn, cucumber, muskmelon and squash, respectively.
Fig (Ficus carica L.) considers the original cultivated fruit trees and currently has become extinct. Such genetic resources should be identified, documented, and conserved. Morphology, pomology, and molecular markers are successful tools in assessing genetic diversity and classifying fig accessions. Twenty-one cultivated fig (F. carica L.) accessions were collected from Egypt and Libya. In Egypt, fig accessions are dispersed from Sinai in the east to El-Saloom in the west and from Alexandria in the north to Aswan in the south, whereas Libyan accessions were collected from Tubryq, Bengazi, and AlKufrah. Seventeen morphological, pomological, and fruit traits were used to characterize the fig accessions. Moreover, frozen young leaves were used to extract genomic DNA; 13 expressed sequence tag (EST) common fig primer pairs with 12 intersimple sequence repeat (ISSR)-anchored primers were used to detect the genetic diversity. Analysis of variance for fig accessions showed highly significant differences concerning morphological traits, i.e., the leaf length (centimeters) and width (centimeters) ranged from 5.4 and 6 cm to 23 and 23.5 cm, for Komesrey-El-Hammam, Abodey-Giza, and Black_Mission accessions, respectively. Also, fig accessions showed different shapes of leaf edge and fruits; they were categorized into four groups: straight, waved, zigzag, and serrated. The number of leaf lobes data ranged from one lobe for the ‘Green-yellow’, ‘Sultani Red Siwa’, and ‘Sultany Red Amria’ accessions to 10 lobes in the Aswany accession. The two-way hierarchical morphological cluster analysis distributed fig accessions into two main groups. The results detected high genetic diversity for the fig accessions that could be useful in the future breeding programs. Concerning molecular data, the EST markers showed highly polymorphism and informative (r = 0.61; 90.0%), with a total number of identified alleles of 78. We proved that a relatively greater number of alleles per locus characterizes the targeted loci among fig accessions, for which only one and two alleles per locus have been revealed, respectively, although ISSR showed a clear pattern and bands of the primers UBC807, UBC811, UBC812, UBC814, UBC815, UBC817, UBC818, and UBC823. In conclusion, a great range of variability was detected within the fig accessions. This diversification could enrich the genetic base of this genus, and more experiments are needed to reach its full potential.
Open-field-grown tomatoes in Saudi Arabia are stressed by poor soil fertility because of the low organic matter content, low nutrient availability, and high pH. Thus, high chemical fertilizer inputs are crucial for commercial production; however, they cause economic loss as well as negatively affect environmental sustainability. The use of efficient organic fertilizers would be useful for developing more cost-effective, environmentally friendly, and sustainable agriculture. The current study evaluated the impact of Black soldier fly (Hermetia illucens L.) frass (BSFF) fertilizer applications as organic fertilizer on postharvest fruit quality and shelf life of open-field-grown F1 hybrid ‘Sinag Tala’ tomatoes compared with those grown under control treatment (received recommended doses of chemical fertilizers for tomato production under open field conditions). The experiment was conducted using a randomized complete block design with four replicates. The results showed that the application of BSFF fertilizer, especially at the higher rate (5 ton/ha; BSFF3), improved the overall quality of tomato fruit. The BSFF fertilizer treatments reduced weight loss, maintained higher firmness, antioxidants [vitamin C, total phenol content (TPC) and total flavinoid (TFC)], total soluble solids (TSS), and titratable acidity (TA) contents, and lowered the TSS/TA ratio of fruits during shelf life compared with those of the control. The decay incidence was only detected after 9 days of shelf life and was significantly lower in all the BSFF fertilizer treatments (range, 8.40%-12.05%) than in the control (15.9%). In addition, BSFF fertilizer treatments reduced both disease incidence and severity of gray mold following pathogen inoculation during shelf life compared with those of the control. These results might be attributable to the higher antioxidants content (vitamin C, TPC, and TFC) and higher polyphenol oxidase activity in BSFF-fertilized tomatoes. In conclusion, BSFF fertilizer could be used as a potential eco-friendly alternative to chemical fertilizers to improve tomato fruit quality during shelf life.