The University of Florida strawberry (Fragaria ×ananassa) breeding population has been continuously improved by recurrent selection since 1968. However, there is a lack of information on genetic parameters that may inform breeding decisions. Parameters were estimated in this population using 19 full-sib families from a 5 × 4 factorial mating design plus six additional biparental crosses and 14 control genotypes including some of the parents. During the 2010–11 season, clonal replicates of the seedling and parental genotypes were distributed within and among two field locations in west–central Florida. Twelve commercially important traits were measured including fruit chemical traits (soluble solids content and titratable acidity), other fruit and yield traits (early and total marketable yields, proportion of total cull fruit, proportion of misshapen fruit, proportion water-damaged fruit, and shape score), and vegetative traits (plant height and total runners). Heritabilities, genotype by environment interaction, and multiple correlations (phenotypic, genotypic, and genetic) were estimated using general mixed model analyses. Narrow-sense heritabilities varied from low to moderate (h 2 = 0.13 ± 0.07 to 0.32 ± 0.09) except for shape score (h 2 = 0.06 ± 0.04) and total average weight (h 2 = 0.52 ± 0.07). Broad-sense heritabilities were larger (H 2 = 0.18 ± 0.03 to 0.53 ± 0.04), and for more than half of the traits, over 50% of the total genetic variation was non-additive. Large genetic and genotypic correlations were found for some traits, most notably between soluble solids content and early marketable yield (–0.68 ± 0.22). Genetic gains for this pair of traits based on a Monte Carlo simulation illustrated the tradeoff between these two traits, showing that a 27% increase in early yield could be obtained through selection but at the expense of an 8% decrease in soluble solids. However, moderate gains can be made in both traits using the appropriate index coefficients.
Vance M. Whitaker, Luis F. Osorio, Tomas Hasing, and Salvador Gezan
Tomas N. Hasing, Luis F. Osorio, and Vance M. Whitaker
Previous studies have recognized considerable variation in the soluble solids content (SSC) of strawberries (Fragaria ×ananassa) during Florida’s fruiting season. Cultivars with stable fruit SSC over time would be valued in the commercial industry as a result of their more uniform quality. The within-season stability of 410 genotypes from the strawberry breeding program of the University of Florida was estimated using data from clonally replicated first-year seedlings and advanced selections evaluated at two locations over two consecutive seasons. Stability was measured using a linear regression approach. For each genotype, the SSC measured at different harvest dates was regressed on the mean SSC of an independent set of genotypes at each harvest date. Genotypes with steep slopes are sensitive to small environmental and physiological changes and are considered unstable compared with genotypes with slopes close to zero. Approximately 90% of individual genotype slopes were not different from the population slope value and were classified in the average stability group, whereas the remaining 10% were equally distributed between the unstable and stable groups. Although a preliminary genetic analysis indicated that SSC stability may have low narrow-sense heritability (h 2 = 0.06 ± 0.05), a group of genotypes exhibited stability across multiple environments. Soluble solids content stability and mean soluble solids were independent, and genotypes with both stable and high levels of SSC were observed.
Colleen Kennedy, Luis F. Osorio, Natalia A. Peres, and Vance M. Whitaker
Powdery mildew (PM) of strawberry (Fragaria sp.) is a ubiquitous, wind-spread disease caused by the obligate parasite Podosphaera aphanis. To control PM, multiple fungicide applications are necessary each season, and none of the major cultivars in Florida have high levels of resistance. Therefore, the objectives of this study were to observe the response to selection and to estimate genetic parameters for PM and related traits in the University of Florida breeding population. In 2010, clonally replicated individuals from seven biparental crosses arising from 11 parents were included in a field trial in which clonally replicated seedlings were evaluated visually for percent coverage of PM mycelium using a modified Horsfall-Barratt scale of 0 to 6. Broad- (H2) and narrow-sense (h2) heritabilities for PM score were (mean ± se) 0.50 ± 0.08 and 0.40 ± 0.39, respectively, for the base population. After the second round of selection in the resistant population, no additive variance was detected, indicating that alleles for PM resistance had become fixed. In contrast, after two rounds of divergent selection in the susceptible population, there remained considerable additive variance (h2 = 0.42 ± 0.65). Moderate to high heritability estimates and a clear response to selection indicate that resistance to PM is genetically controlled through mostly additive effects. Selection of parents based on field trials with natural inoculum should result in good progress toward more resistant cultivars. The consistently moderate to strong genotypic and genetic correlations among PM and canopy density (CD) indicate that selection for PM resistance will result in reduced CD. Therefore, CD must be monitored over successive rounds of selection for low levels of PM to prevent CD falling below the commercially acceptable range.