Search Results
You are looking at 1 - 2 of 2 items for :
- Author or Editor: Ling Wang x
- Journal of the American Society for Horticultural Science x
The physiological role of arginase in nitrogen remobilization processes from protein degradation during seed germination has well been described in several species. However, very little is known about its possible roles in plant stress responses. Treatment of tomato fruit (Solanum lycopersicum L.) with 0.05 mm methyl jasmonate (MeJA) enhanced transcription levels of arginase genes, especially LeARG2. Chilling injury (CI) of fruit treated with 0.05 mm MeJA for 12 hours was also effectively alleviated, as manifested by decreases in CI index, electrolyte leakage, and malondialdehyde (MDA) content. To investigate the potential role of arginase in MeJA-induced chilling tolerance, fruit were treated with MeJA or the arginase inhibitor Nω-hydroxy-nor-l-arginine (nor-NOHA) combined with MeJA and then stored at 2 °C for 28 days. MeJA-induced arginase activity was strongly inhibited and the reduction of CI by MeJA was nearly abolished by the inhibitor. In addition, MeJA treatment increased the activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX); inhibited peroxidase (POD) activities; and promoted proline and polyamines accumulation. These effects were partially counteracted by nor-NOHA; putrescine accumulation, however, was unaffected by the inhibitor. Our results indicate that arginase may be involved in MeJA-induced chilling tolerance, possibly by ameliorating the antioxidant enzyme system of fruit and increasing proline levels.
Chinese Artichoke (Stachys sieboldii Miq.), an indigenous vegetable in China, is renowned for its rich nutritional content and medicinal properties. With a significant cultivation history and promising prospects, this vegetable holds great potential for further development. In this study, we investigated changes in gene expression levels and metabolite content within relevant metabolic pathways during the development of Chinese Artichoke tubers. Tubers from three different periods—S1 (6 Aug), S2 (2 Sep), and S3 (1 Oct)—served as materials for the analysis. The results revealed that differentially expressed genes and differentially accumulated metabolites were predominantly enriched in pathways associated with secondary metabolite synthesis, carbohydrate metabolism, amino acid metabolism, and lipid metabolism. These pathways are known to play significant regulatory roles in the development of Chinese Artichoke tubers. Two crucial metabolic pathways identified through a comprehensive analysis of transcriptomics and metabolomics were further investigated. The genes GOLS, STS, AGAL, and INV potentially play crucial roles in stachyose accumulation. Additionally, the genes trpB (Cluster-5100.0), GOT2 (Cluster-7051.0), and TYRAAT (Cluster-4023.14777) potentially influence the aromatic amino acid content at different developmental stages. However, the regulatory mechanisms of these genes and their relationship with metabolites require further investigation. The study findings provide a theoretical foundation for understanding the key pathways that influence the growth of Chinese Artichoke tubers, metabolic mechanisms of stachyose and amino acids, verification of gene functions, and genetic enhancement of Chinese Artichoke cultivars.