Drought stress is one of the major limiting factors for plant growth and development. The mechanism of drought tolerance has not been well understood. This study was designed to investigate proline and antioxidant metabolism associated with drought tolerance in transgenic tobacco (Nicotiana tabacum) plants overexpressing the OjERF gene relative to wild-type (WT) plants. The OjERF gene was isolated from mondo grass (Ophiopogon japonicus). The OjERF gene, driven by the CaMV35S promoter, was introduced into tobacco through agrobacterium (Agrobacterium tumefaciens)-mediated transformation. Five transgenic lines were regenerated, of which transgenic Line 5 (GT5) and Line 6 (GT6) were used to examine drought tolerance in comparison with WT plants in a growth chamber. Drought stress caused an increase in leaf malondialdehyde (MDA) and electrolyte leakage (EL), proline content, superoxide dismutase (SOD), and catalase (CAT) activity in both transgenic lines and WT plants. However, the transgenic lines had lower MDA content and EL and higher proline content, SOD and CAT activity relative to WT under drought stress. The activities of SOD and CAT were also greater in the transgenic lines relative to WT plants under well-watered conditions (Day 0). The OjERF activated the expression of stress-relative genes, including NtERD10B, NtERD10C, NtERF5, NtSOD, and NtCAT1 in tobacco plants. The results of this study suggest that the OjERF gene may confer drought stress tolerance through upregulating proline and antioxidant metabolism.
Cong Li, Lie-Bao Han and Xunzhong Zhang
Cheng-lie Zhang, Paul H. Li and Charles C. Shin
Twenty-day-old `Bush Blue Lake 47' common bean plants grown in a growth chamber at 25 days/22C night and a 12-hour photoperiod regime were foliar sprayed with 0.5% GLK-8903 including 0.05% Tween-20. After 24 hours of treatment, plants were chilled in a cold room (4C day/night, 12 hours of light). After 3 days of chilling, leaves of untreated controls were injured, as visually characterized by leaf wilting, whereas leaves of the GLK-8903-treated plants still retained turgor. During chilling, the activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) decreased. GLK-8903 treatment had no effect on SOD and POD activities; however, the CAT activity was reduced significantly after GLK-8903 treatment either at 25 or at 4C. During chilling, the content of malondialdehyde, a decomposition product of phospholipid peroxidation, increased in treated plants and untreated controls, with increased content significantly lower in the former compared with the latter. The GLK-8903 per se and total lipid extracted from GLK-8903-treated plants were able to reduce the linoleic acid oxidation in vitro. The mechanism by which GLK-8903 alleviates chilling injury in bean plants is discussed.
Li-Juan Zhang, Tian-Xiu Zhong, Li-Xin Xu, Lie-bao Han and Xunzhong Zhang
Soil water deficit impacts cold acclimation and freezing tolerance in creeping bentgrass (Agrostis stolonifera L.), but the mechanisms underlying have not been well understood. The objectives of this study were to investigate the effects of deficit irrigation before and during cold acclimation on osmoprotectants, antioxidant metabolism, and freezing tolerance in creeping bentgrass. The grass was subjected to three-soil moisture levels: well-watered [100% container capacity (CC)], deficit irrigation induced-mild drought stress (60% CC), and severe drought stress (30% CC) for 35 days including 14 days at 24/20 °C (day/night) and then 21 days under cold acclimation treatment (2 °C) in growth chambers. Leaf proline and total soluble sugar (TSS) levels were higher in the grass under mild drought stress relative to that under severe drought stress. Superoxide (O2 −·), hydrogen peroxide (H2O2), and malondialdehyde (MDA) content were higher in the grass under severe drought relative to that under well-watered and mild drought stress at day 35. Mild drought stress increased catalase (CAT) and guaiacol peroxidase (POD) activity, induced new isoforms and increased band intensities of superoxide dismutase (SOD), CAT, and POD during cold acclimation (days 14 to 35). No differences in osmoprotectants, antioxidant metabolism, and freezing tolerance were found between mild drought and well-watered treatments. The results of this study suggest deficit irrigation-induced mild drought stress in late fall and winter could induce accumulation of osmoprotectants and improve antioxidant metabolism, and freezing tolerance, but severe drought stress could reduce freezing tolerance of creeping bentgrass in the region with limited precipitation.
Lie Li, Yu-xin Tong, Jun-ling Lu, Yang-mei Li and Qi-chang Yang
Light, as the energy and signal sources for plant growth and development, is one of the most important environment factors in recently developed plant factories with artificial light (PFALs). To find the optimal combination of light wavelengths for lettuce (Lactuca sativa cv. ‘Tiberius’) plant growth in a PFAL, four treatments, each using red (R; 662 nm) and blue light (B; 447 nm) with a ratio of 4:1 and photon flux density (PFD) of 150 μmol·m−2·s−1, and mixing, respectively, with 50 μmol·m−2·s−1 of green light (G; 525 nm; RBG), yellow light (Y; 592 nm; RBY), orange light (O; 605 nm; RBO) and far-red light (FR; 742 nm; RBFR), were set up during this experiment. A combination of R and B with a ratio of 4:1 and PFD of 200 μmol·m−2·s−1 was set as the control (RB). The responses of lettuce growth, morphology, anatomical structure of the lettuce leaf, photosynthetic performance, lettuce nutritional quality, and energy use efficiency were investigated. The results showed that RBG, RBO, and RBFR increased the shoot fresh weight of lettuce by 20.5%, 19.6%, and 40.4%, and they increased the shoot dry weight of lettuce by 24.2%, 13.4%, and 45.2%, respectively, compared with those under RB. The Pn under RBY was significantly lower than that under RB, although no significant differences in chlorophyll or carotenoid content were found between RBY and RB. RBG increased the lettuce leaf area, the thickness of the leaf palisade tissue, Pn, and light use efficiency compared with those under RB. Plants grown under RBO showed better photosynthetic capacity, such as higher Pn, ΦPSII, and other photosynthetic parameters. RBFR caused an increase in lettuce leaf area and energy use efficiency, but a decrease in leaf thickness and Pn of the single leaf. Moreover, tipburn injury was observed under RBFR. Therefore, these results demonstrate that RBG and RBO can be considered optimal combinations of light wavelengths for lettuce growth in a PFAL in this experiment, although plant growth can also be improved by using RBFR.