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Open access

Leonard P. Stoltz

Abstract

A cattleya orchid clone (Cattleya labiata. Lindl.) grown in vitro at 7 concentrations of iron chelate prepared according to the method Steiner and van Winden at 0, 0.5, 1, 5, 10, 20, 40 ppm showed the most growth at 0 to 10 ppm and reduced growth at 20 and 40 ppm. Iron concentration of the tissue was 486 ppm at the beginning of the study and varied from 33 to 2880 ppm on dry weight basis at the end of 17 weeks. The number of propagules at the end of the study varied with tissue fresh weight.

Open access

Leonard P. Stoltz

Abstract

Excised mature Iris embryos were grown on a nutrient medium with various agar concentrations. Fresh wt, root, and shoot length were inversely related to the agar concentration. Methods for measuring certain physical effects of agar were devised. Agar added to nutrient media is considered to reduce water availability to tissues growing on it by binding of water.

Open access

Leonard P. Stoltz

Abstract

Hydrangea macrophylla Thunb. ‘Merveille’ can be propagated by tissue culture and the resulting plants acclimated to a greenhouse environment. Utilizing rapidly replicating cultures for continued propagation and slow replicating cultures for producing plants in a greenhouse is an effective method of increasing the overall tissue culture propagation rate. Planting out in a greenhouse by 30 July at the latitude of Lexington, Ky., provided adequate time to develop hydrangea plants that produced 3 or more flowers per plant by 15 Apr.

Open access

Leonard P. Stoltz

Abstract

In a study of nutrient requirements of American ginseng (Panax quinquefolium L.) foliar deficiency symptoms of Ca occurred first, followed by those for Mg and P. N-deficiency symptoms occurred very gradually, and S-deficiency symptoms never appeared. Root fresh weight gain was most restricted by omission of either Ca, P, or Mg from the nutrient solution. Omission of S resulted in a root fresh weight gain equal to that obtained with complete nutrient solution.

Open access

Leonard P. Stoltz

Abstract

Excised mature embryos of iris (Iris sp.) were grown on agar nutrient medium with 6 concentrations each of indolebutyric acid (IBA), naphthalene acetic acid (NAA), 2,4-dichlorophenoxy-acetic acid, (2,4-D), gibberellic acid (GA3), and kinetin (Kn). The number of roots occurring on the seedlings was significantly increased by 10-5 and 5 × 10-5M IBA and 5 × 10-7, 10-6 and 5 × 10-6M NAA. Plant tissue weight was significantly increased by 10-6 to 10-5M NAA, 10-5 and 5 × 10-5M IBA and 5 × 10-7M 2,4-D. Number of leaves per seedling was most affected by Kn and GA3 levels of 10-5 and 5 × 10-5M. Root length was longest at 10-7M Kn and 5 × 10-7, 10-6 and 5 × 10-6M GA3.

Open access

Leonard P. Stoltz

Abstract

The most effective medium for in vitro propagation of Acalypha wilkesiana Mull. Agr. was composed of Murashige and Skoog mineral salts and vitamins, 2% sucrose, 170 mg/liter NaH2PO4·H2O and 0.2 mg/liter benzylamino purine (BA) without auxin. A modified White’s medium without auxin gave good rooting in 10 to 14 days. Plantlets planted into soil in the greenhouse were ready for transplanting outdoors in 45 days.

Free access

Baolin Zhang and Leonard P. Stoltz

Open access

Leonard P. Stoltz and John C. Snyder

Abstract

Embryos of American ginseng (Panax quinquifolius L.) seeds stratified 570 days at 0° or 5°C did not increase in size, whereas embryos of seeds stratified 570 days at 20° grew to a length of 2.5 mm. Embryos of seeds stratified at 5° for 120 days, treated with 1000 ppm gibberellic acid (GA), then transferred to 20° grew to the same length as those held at a constant 20°. None of the GA treated seeds germinated. However, seeds stratified outdoors and those provided 5° for 120 days, 20° for 300 days, and then held at 5°C germinated after 540 days. The embryos of these latter treatments had similar growth patterns. Growth of the cotyledons paralleled the growth of the entire embryo. Cool-warm-cool stratification patterns are necessary for efficacious germination of American ginseng seeds.

Open access

Jennifer M. Kiernan, James W. Hendrix, Leonard P. Stoltz, and Dale M. Maronek

Abstract

Tissue-culture-produced nonmycorrhizal strawberry plants (Fragaria x awanassa Duchesne ‘Guardian’) were inoculated with spores of the endogonaceous mycorrhizal fungi Glomus mosseae, G. epigaeum, or G. constrictum. The plants were grown for 14 weeks in the greenhouse in a steamed 1 peat: 1 perlite mixture (v:v) containing a slow release fertilizer, 18-6-12 Osmocote (18N-2.6P-10K), 9-month release rate. Inoculated plants were slightly larger than noninoculated control plants. Roots were colonized more by G. mosseae than by G. epigaeum. No evidence of root colonization by G. constrictum was found, but the fungus sporulated.