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  • Author or Editor: Ledare S. Finley x
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Flowering dogwood (Cornus florida L.) is an important tree of forests and urban landscapes in the eastern United States. Amplified fragment length polymorphism (AFLP) markers were generated from genomic DNA of 17 cultivars and lines, and four duplicate samples of selective cultivars. Specific markers were identified for all except the following two lines and cultivar: MW94-67, MW95-12, and ‘Plena’. A dichotomous cultivar identification key was constructed based on AFLP data, and specific peaks or combinations of peaks were identified for all cultivars and lines. The key was assessed with seven anonymous (unlabeled) dogwood samples, and all unknowns except one were identified using the dichotomous key. Two of the unknown samples, ‘Cherokee Chief’ and ‘Cherokee Brave’, were difficult to distinguish using the AFLP markers. Intracultivar variation, up to 36% dissimilarity, was observed between duplicate samples of the same cultivar from different trees, suggesting that some mislabeling of trees had occurred at the nursery. The cultivar-specific AFLP markers can be used in breeding applications, patent protection, and in future projects, such as mapping the C. florida genome.

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