Commercially available biocontrol agents Trichoderma harzianum Rifai and the arbuscular mycorrhizal fungus Glomus intraradices Schenck and Smith were tested for their efficacy in controlling fusarium root rot in potted asparagus (Asparagus officinalis L.) seedlings. High and low concentrations of Fusarium oxysporum (Schlect.) emend. Snyd. & Hans. f. sp. asparagi Cohen & Heald (FOA) were combined with G. intraradices and/or T. harzianum treatments. In both experiments included in this study, T. harzianum and G. intraradices alone and in combination effectively reduced root rot caused by FOA when asparagus seedlings were grown in low levels of FOA-infested medium. When seedlings were grown in high levels of FOA-infested medium, the combination of T. harzianum + G. intraradices significantly increased dry shoot mass and limited root rot compared to the control.