Diploid populations and progenies of controlled crosses of blueberry, Vaccinium section Cyanococcus, were analyzed by starch gel electrophoresis for nine enzyme systems, aconitase (ACO), aldolase (ALD), alcohol dehydrogenase (ADH), glyceraldehyde-3 -phosphate dehydrogenase (G-3-PDH), isocitrate dehydrogenase (IDH), leucine aminopeptidase (LAP), malate dehydrogenase (MDH), 6-phosphogluconate dehydrogenase (6-PGD), and phosphoglucose isomerase (PGI). Allozyme variants indicated the existence of three alleles at Ace-1, Ald, G-3-pdh-2, and Mdh-2; four alleles at Mdh-3 and 6-Pgd-2; five alleles at Aco-2, Idh, Lap-1, and 6-Pgd-1; six alleles at Adh-2 and Pgm-2; and nine alleles at Pgi-2. In addition, a null allele was found at Lap-1. In the majority of progenies, the inheritance patterns for each of these loci were consistent with mendelian laws for single gene control. Forty-seven pairs of loci were tested for independent assortment revealing two linked pairs, Pgi-2/Lap-1 and Pgm-2/6-Pgd-2, which appeared to represent two independent linkage groups.