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  • Author or Editor: L.-J. Chen x
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Abstract

Plantlets orginating from adventitious buds of explants obtained from the decapitated shoot apex of a banana sucker established well under field conditions and gave rise to mature plants with uniform growth and normal yield of fruit. A total of one million pathogen-free plantlets for commercial planting was produced in 1983. Introduction of plantlets for commercial planting prevents the spreading of fusarial wilt of banana by planting materials to the disease-free orchards.

Open Access

Proponents of global climate models predict a doubling of world CO2 concentration from 350 to 600 ppm by the year 2030, concurrent with a 2-5°C temperature increase. Consequences of this “greenhouse effect” on Oryza sativa L. were determined using four rice lines selected for their widespread use in cultivation and research. A 2×2 factorial design was used with CO2 at 350 and 600 ppm and day/night temperature regimes of 31/27°C and 37/33°C. Combined effects of CO2/temperature were determined during 5 harvests from seeding to reproductive maturity. Elevated CO2 enhanced dry weight and photosynthetic capacity over both temperature regimes relative to plants grown at ambient CO2. The 37/33°C day/night temperature regime increased sterility in rice by decreasing pollen shed.

Free access

Abstract

Hydrogen cyanamide or hot-water treatment (47C) for 1 hr at the 250°GS (Growth Stage) effectively broke rest in dogwood buds within 10 to 12 days. At this growth stage, control plants grown at 25/18C (day/night) maintained an LT50 of –25C throughout the 3-week study period, whereas plants treated with H2CN2 or hot water gradually lost hardiness. After 3 weeks at 5/2C (day/night), the control plants hardened to a hardiness (LT50) of –45C, and H2CN2-treated plants maintained an LT50 of –25C. The results demonstrate that the extent of acclimation and deacclimaton of dogwood plants may be influenced by environmental temperatures and rest status.

Open Access

Two cultivars of Anthurium andraeanum Hort. hybrids, `Paradise Pink' and `Tropic Flame', were transformed by Agrobacterium to contain gene sequences for Shiva-1, a cecropin-based lytic peptide. The antibacterial gene was driven by a 35-35S cauliflower mosaic viral (35-35S CaMV) promoter and the construct included the secretory signal sequence for pathogenesis-related protein 1b (PR1b). Blight tolerance of regenerated plants was tested by inoculation with a virulent strain of Xanthomonas axonopodis (formerly campestris) pv. dieffenbachiae (Xad) that is bioluminescent to allow detection of symptomless infections in Shiva-1 transformants. Primary regenerants for two Shiva-1 transgenic lines of `Paradise Pink' displayed significantly enhanced tolerance to bacterial blight over blight susceptible `Rudolph' and even the blight tolerant `Kalapana'. Two Shiva-1 transgenic lines of `Tropic Flame' showed no improved resistance when compared to the control at the mean percent leaf infection level. One Shiva-1 transgenic line of `Tropic Flame' was unexpectedly more susceptible to blight than the nontransgenic control. Low expression of Shiva-1 observed in this line is hypothesized to be the cause of its increased susceptibility to Xad.

Free access