Oviposition and feeding of Scrobipalpuloides absoluta was studied on plants of seven different genotypes with varying 2-tridecanone (2-TD) foliage concentrations: Lycopersicon esculentum var. glabratum PI 1344417 (GLA), L. esculentum TSWV-547 (ESC), F1 (ESC × GLA), and four F2 genotypes—two with high 2-TD (HI1, HI2) and two with low foliage 2-TD concentrations (LO1, LO2). GLA, HI1, and HI2 showed 2-TD concentrations above 185 × 10–12 mol·cm2, while ESC, LO1 and LO2 had 2-TD below 40 × 10–12 mol·cm2; F1 had intermediary levels of 2-TD (83.5 × 10–12 mol 2-TD/cm2). Ovipositioning was substantially higher in the low 2-TD than on either the high 2-TD genotypes or in the F1, especially in the upper portion of the plants. Scores for leaf lesion type (LLT), overall plant damage (OPD) and percent leaflets attacked (PLA) were substantially higher for the low 2-TD than for either the high 2-TD genotypes or the F1. The results indicate that 2-TD mediates resistance to Scrobipalpuloides absoluta in the interspecific cross, and strongly suggest that 2-TD acts as both an ovipositioning and feeding deterrent for this insect.